Integrated nucleic acid detection cassette for chlamydia trachomatis

Abstract

The invention discloses an integrated nucleic acid detection cassette for chlamydia trachomatis, which comprises a cassette body with a cassette cover at the top, a lysis cavity, a first cleaning cavity and a second cleaning cavity are sequentially arranged in the cassette body from top to bottom, the lysis cavity is communicated to the cassette cover, and the second cleaning cavity is communicated to a reaction cavity arranged at the bottom of the outer side of the cassette body; plungers with the plunger hole direction perpendicular to the connecting direction are arranged in the pairwise connecting intervals of the cracking cavity, the first cleaning cavity, the second cleaning cavity and the reaction cavity respectively, and hydrophobic liquid sealing materials are arranged in the plunger holes of the plungers; and an amplification reaction solution is arranged in the reaction cavity, a soluble material layer is arranged in the reaction cavity, and a primer probe mixed solution for Aomp gene of chlamydia trachomatis is embedded in the soluble material layer. According to the invention, the chlamydia trachomatis can be rapidly and integrally subjected to PCR amplification and detection under the condition of relatively low cost, and the problems of poor repeatability, time consumption of a detection method, complicated operation and the like in the prior art are solved.

Description

technical field [0001] The invention relates to an integrated nucleic acid detection cartridge for Chlamydia trachomatis, which belongs to the technical field of medical detection. Background technique [0002] Chlamydia trachomatis (CT) is a class of intracellular parasitic microorganisms, about 250-450 nm in size, gram-negative, round or oval; it contains DNA, RNA and ribosomes; it has a cell wall, and its structure and composition are similar in Gram-negative bacteria. Chlamydia trachomatis is divided into 12 serotypes based on serotyping of monoclonal or polyclonal antibodies against outer membrane proteins: A-K. Among them, A, B / Ba and C types mainly cause trachoma; D-K types mainly cause urogenital tract infection. [0003] Chlamydia trachomatis (CT) can cause non-gonococcal urethritis and pelvic inflammatory disease. Chlamydia trachomatis infection shows a series of clinical symptoms, such as urethritis, cervicitis, prostatitis, venereal lymphogranuloma, Reiter syn...

AI Technical Summary

Problems solved by technology

It can perform rapid and integrated PCR amplification and detection of Chlamydia trachomatis at a relatively low cost, and solve the problems of poor repeatability of the current technology, time-consuming detection methods, and complicated operations.

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