Integrated nucleic acid detection card box for canine adenovirus

Abstract

The invention discloses an integrated nucleic acid detection card box for a canine adenovirus. The integrated nucleic acid detection card box comprises a box body with a box cover at the top, wherein a cracking cavity, a first cleaning cavity and a second cleaning cavity are sequentially arranged in the box body from top to bottom, the cracking cavity communicates with the box cover, and the second cleaning cavity communicates with a reaction cavity arranged at the bottom of the outer side of the box body; plungers of which the plunger hole directions are perpendicular to the connecting direction are respectively arranged on the pairwise connected regions of the cracking cavity, the first cleaning cavity, the second cleaning cavity and the reaction cavity, and hydrophobic liquid sealing materials are arranged in plunger holes of the plungers; and amplification reaction liquid is arranged in the reaction cavity, a soluble material layer is arranged in the reaction cavity, and primer probe mixed liquid of a canine adenovirus I type hexon gene and a canine adenovirus II type hexon gene is embedded in the soluble material layer. According to the invention, PCR amplification and detection can be rapidly and integrally carried out on the canine adenovirus under the condition of relatively low cost, and the problems of poor repeatability, time-consuming detection method, complex operation and the like in the prior art are solved.

Description

technical field [0001] The invention relates to a canine adenovirus integrated nucleic acid detection cartridge, which belongs to the technical field of medical detection. Background technique [0002] Canine adenovirus is an animal virus with the strongest pathogenicity and well-studied morphological structure among known mammalian adenoviruses. There are two serotypes of canine adenovirus (CAV): CAV-1 and CAV-2. CAV-1 is the pathogen of infectious canine hepatitis, which is one of the three major infectious diseases in canine animal diseases, which are juxtaposed with canine distemper and viral enteritis. Canine adenovirus type 2 (CAV-2) is one of the causes related to the pathogenesis of canine infectious tracheobronchitis (ITB), which can cause mild acute upper respiratory tract disease in dogs. Canine infectious bronchitis is a respiratory infectious disease, which is highly contagious and occurs in groups, which is very harmful to dogs. [0003] Canine adenovirus, l...

AI Technical Summary

Problems solved by technology

It can quickly and integratedly perform PCR amplification, detection and type identification on canine adenovirus at a relatively low cost, and solve the problems of poor repeatability of the current technology, time-consuming detection methods, and complicated operations.

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