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Autologous fat multi-component full-automatic separation, preparation and extraction device and method thereof

An extraction device and fully automatic technology, which is applied in the field of biomedical engineering, can solve the problems of low automation means and low utilization rate, and achieve the effects of high extraction efficiency, reduced possibility of pollution, and complete types

Active Publication Date: 2021-05-11
HANGZHOU BAIQIAO MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the utilization rate of autologous fat is not high when separating and extracting the above-mentioned active ingredients, and the automatic method of extraction is low, which cannot meet the clinical needs

Method used

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  • Autologous fat multi-component full-automatic separation, preparation and extraction device and method thereof
  • Autologous fat multi-component full-automatic separation, preparation and extraction device and method thereof
  • Autologous fat multi-component full-automatic separation, preparation and extraction device and method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Such as Figure 1-20 As shown, the autologous fat multi-component automatic separation, preparation and extraction device includes a dry-wet separation unit 10, a fat crushing and cell extraction unit 20, an exosome preparation unit 30 and a centrifugal extraction unit 50, such as figure 1 As shown, the structure diagram of the corresponding unit is in the bottom box, which is convenient for corresponding understanding with the expanded description of each unit below.

[0064] Such as Figure 2-7 As shown, the dry-wet separation unit 10 in this embodiment includes a separation box 11, and an upper cover 12 is fastened on the separation box 11. The separation box 11 and the upper cover 12 form a sealed box, which can be a square box. Two filter boxes are placed in the separation box 11 from top to bottom, namely the primary filter box 13 and the secondary filter box 14, and the separation box 11 is divided into three sections. In this embodiment, the primary filter box...

Embodiment 2

[0104] Such as Figure 14-18 As shown, based on Example 1, the difference between the autologous fat multi-component automatic separation, preparation and extraction device in this embodiment is that only the exosome preparation unit 30 is replaced, and the exosome preparation unit 30 adopts positive pressure filtration. Filtration. During the filtration process, the internal pressure can not only be kept constant, but also the filter membrane can be flushed with reciprocating flow to improve the filtration effect and shorten the filtration time. It can extract a large amount of exosomes and is suitable for commercial mass production.

[0105] In this embodiment, the exosome preparation unit 30 is a device for performing two-stage filtration of exosomes. Specifically, the exosome preparation unit 30 includes a bottom plate 443 on which a square casing and three tank holders 421 located outside the square casing and distributed side by side from left to right are installed on t...

Embodiment 3

[0113] Such as Figure 23 As shown, a multi-component automatic separation, preparation and extraction method of autologous fat, based on embodiment 1 or embodiment 2, is the process flow of the automatic multi-component separation, preparation and extraction method of autologous fat, and the multi-component automatic autologous fat The separation, preparation and extraction method comprises the following steps:

[0114] Step 1. Open the upper cover 12 of the dry-wet separation unit 10, inject the sucked autologous fat into the uppermost filter box, and separate the sucked autologous fat through different filter boxes to obtain connective tissue, Fats and mixed liquids.

[0115] Step 2. Put the connective tissue or fat obtained in step 1 into the second storage tank 227 of the fat crushing and cell extraction unit 20, start the power supply, and the cleaning solution in the first storage tank 226 is pumped in the first plunger pump 206 Enter the second storage tank 227 under...

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Abstract

The invention discloses an autologous fat multi-component full-automatic separation, preparation and extraction device and method. The autologous fat multi-component full-automatic separation, preparation and extraction device comprises a dry-wet separation unit, a fat disruption and cell extraction unit, an exosome preparation unit and a centrifugal extraction unit. According to the full-automatic separation, preparation and extraction method for multiple components of autologous fat, connective tissues, fat and mixed liquid are obtained by the dry-wet separation unit, and filling particles, fat particles, adipose stem cells or vascular matrix components are obtained by the fat crushing and cell extraction unit; an exosome concentrated solution is obtained from the mixed liquid or an exosome culture solution obtained from adipose-derived stem cells by the exosome preparation unit, and the exosome concentrated solution is centrifugally separated by the centrifugal extraction unit and then is sucked and sampled. The method is complete in prepared and extracted component variety, high in extraction efficiency, high in automation degree, capable of efficiently utilizing autologous fat, high in extracted component activity, low in pollution, and suitable for clinical application or commercial mass production.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a device and method for fully automatic separation, preparation and extraction of autologous fat multi-components. Background technique [0002] Due to the advantages of high cure rate, low immune rejection, long retention time, and good cosmetic effect in cell therapy, plastic repair, wrinkle removal, breast augmentation and other cosmetic operations, the autologous fat sucked for slimming and shaping has become more and more widely used. . Autologous fat includes important components such as fat particles, adipose-derived stem cells (ADSCs), stromal vascular fraction (SVF), and exosomes. Among them, fat particles have an important application in filling cosmetic surgery, such as breast augmentation surgery. Adipose-derived stem cells (ADSCs) play an important role in building cell-material complexes for prosthetic modification. Stromal vascular fraction (SVF...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D36/02
CPCB01D36/02
Inventor 李宏李博文
Owner HANGZHOU BAIQIAO MEDICAL TECH CO LTD
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