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Probiotic fermentation type eucommia ulmoides fruit and vegetable freeze-dried powder and preparation method thereof

A fermentative, probiotic technology with applications in biochemical equipment and methods, microbe-based methods, bacteria used in food preparation, etc.

Pending Publication Date: 2021-05-11
河南清大生命健康产业技术研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no eucommia probiotic powder product yet, especially it can improve the taste of eucommia itself, and reduce the unpleasant taste of traditional Chinese medicine brought by eucommia leaves.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] MRS liquid medium formula: peptone 10 g / L, yeast extract 5 g / L, diammonium citrate 2 g / L, glucose 20 g / L, beef extract 10 g / L, Tween 80 0.1 g / L, sulfuric acid Magnesium 0.58 g / L, manganese sulfate 0.28 g / L, dipotassium hydrogen phosphate 2 g / L, pH 6.4.

[0022] MRS solid medium formula: peptone 10 g / L, yeast extract 5 g / L, diammonium citrate 2 g / L, glucose 20 g / L, beef extract 10 g / L, Tween 80 0.1 g / L, magnesium sulfate 0.58 g / L, manganese sulfate 0.28 g / L, dipotassium hydrogen phosphate 2 g / L, 15 g / L agar, pH6.4.

[0023] A preparation method of probiotic fermented eucommia fruit and vegetable freeze-dried powder, the steps comprising:

[0024] (1) Preparation of seed liquid: pick single colonies of activated Lactobacillus reuteri, Lactobacillus plantarum, and Lactobacillus bulgaricus from the MRS solid medium, and place them in the corresponding prepared MRS liquid medium, and culture them at 30°C for 15 h , collected by centrifugation, and diluted to an OD600 value...

Embodiment 2

[0030] MRS liquid medium formula: peptone 10 g / L, yeast extract 5 g / L, diammonium citrate 2 g / L, glucose 20 g / L, beef extract 10 g / L, Tween 80 0.1 g / L, sulfuric acid Magnesium 0.58 g / L, manganese sulfate 0.28 g / L, dipotassium hydrogen phosphate 2 g / L, pH 6.4.

[0031] MRS solid medium formula: peptone 10 g / L, yeast extract 5 g / L, diammonium citrate 2 g / L, glucose 20 g / L, beef extract 10 g / L, Tween 80 0.1 g / L, magnesium sulfate 0.58 g / L, manganese sulfate 0.28 g / L, dipotassium hydrogen phosphate 2 g / L, 15 g / L agar, pH6.4.

[0032] A preparation method of probiotic fermented eucommia fruit and vegetable freeze-dried powder, the steps comprising:

[0033] (1) Preparation of seed liquid: pick single colonies of activated Lactobacillus reuteri, Lactobacillus plantarum, and Lactobacillus bulgaricus from the MRS solid medium, and place them in the corresponding prepared MRS liquid medium, and culture them at 30°C for 15 h , collected by centrifugation, and diluted to an OD600 value...

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PUM

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Abstract

The invention provides probiotic fermentation type eucommia ulmoides fruit and vegetable freeze-dried powder. The freeze-dried powder is prepared by fermenting lactobacillus reuteri, lactobacillus plantarum and lactobacillus bulgaricus in a eucommia ulmoides fruit and vegetable culture medium and then performing freeze drying, wherein the viable counts of the lactobacillus reuteri, the lactobacillus plantarum and the lactobacillus bulgaricus are all 10<7>-10<9>cfu / g; and the eucommia ulmoides fruit and vegetable culture medium is prepared by pulping tomatoes, carrots, peaches, eucommia ulmoides leaves and edible glucose. According to the freeze-dried powder, the utilization of effective components of the eucommia ulmoides leaves is greatly improved, and the unpleasant traditional Chinese medicine taste brought by the eucommia ulmoides leaves is greatly relieved.

Description

technical field [0001] The invention belongs to the technical field of food processing, and in particular relates to a probiotic fermented eucommia fruit and vegetable freeze-dried powder and a preparation method thereof. Background technique [0002] Probiotics can normally exert metabolic activity in the body, decompose protein, sugar, synthetic vitamins and fats, small molecule peptides and essential amino acids in food, significantly improve the digestibility and biological value of food, and promote gastrointestinal absorption. [0003] Eucommia Ulmoides Oliv. is a deciduous tree of the genus Eucommia in the family Eucommia. It is a rare and endangered second-class protected plant and a national strategic tree species unique to China. The dry bark of Eucommia is a valuable Chinese tonic. It is sweet in taste and warm in nature. It has the effects of nourishing liver and kidney, strengthening tendons and bones, regulating Chong Ren, consolidating meridian and preventin...

Claims

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Application Information

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IPC IPC(8): A23L19/00A23L33/00A23L33/135C12N1/20C12R1/225C12R1/25
CPCA23L19/01A23L33/00A23L33/135C12N1/20A23V2002/00A23V2400/123A23V2400/173A23V2400/169A23V2200/30A23V2200/3204A23V2200/324
Inventor 王立言沈小娟许言
Owner 河南清大生命健康产业技术研究院有限公司
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