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Lipoprotein (a) determination kit and detection method thereof

A kit and lipoprotein technology, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as difficult promotion and use in grassroots hospitals, high cost of measurement, unsatisfactory sensitivity and precision, and anti-interference ability, etc., to achieve The effect of high degree of automation, small amount of detection samples, and improvement of work efficiency

Active Publication Date: 2021-05-07
广州市伊川生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the characteristics of its light wave receiving method, the sensitivity and speed of the test results are usually better than those of the transmission turbidimetry. However, it requires a special nephelometry instrument or a special protein meter, and special matching reagents. High, not easy to promote and use in primary hospitals
Turbidimetric transmission (ITA) method is easy to operate and has strong applicability. Both ordinary automatic biochemical analyzers and spectrophotometers can be used, and it is easier to be used for routine analysis. Almost all laboratories and primary hospitals can carry out it. The sensitivity, precision and anti-interference ability are not ideal enough

Method used

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  • Lipoprotein (a) determination kit and detection method thereof
  • Lipoprotein (a) determination kit and detection method thereof
  • Lipoprotein (a) determination kit and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The preparation of embodiment 1 lipoprotein (a) detection kit

[0037] Reagent 1 (R1) is:

[0038]

[0039]

[0040] Anti-interference agent:

[0041] Lauryl dimethyl betaine 15g / L

[0042] Triton X-100 5g / L

[0043] Reagent 2 (R2) is:

[0044]

[0045] The preparation method of human lipoprotein (a) monoclonal antibody latex microspheres is as follows:

[0046] (1) Take 1 mL of carboxylated latex microspheres (150 nm, 10% solid content) and dilute to 5 mL with glycine buffer.

[0047] (2) Add 1.5ml 25g / L N-hydroxysuccinimide (NHS) and 1.5ml 9.0g / L 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt Hydrochloric acid (EDC) was activated, stirred evenly and incubated at 37°C for 20min.

[0048] (3) Add 3 mL of phosphate buffer solution and stir well at room temperature.

[0049] (4) Add 2 g of goat anti-human lipoprotein (a) monoclonal antibody and incubate at 37° C. for 4 hours.

[0050] (5) Add 3 mL of stop solution to terminate the reaction, stir at r...

Embodiment 2

[0052] The preparation of embodiment 2 lipoprotein (a) detection kit

[0053] Reagent 1 (R1) is:

[0054]

[0055] Reagent 2 (R2) is:

[0056]

Embodiment 3

[0057] The preparation of embodiment 3 lipoprotein (a) detection kit

[0058] Reagent 1 (R1) is:

[0059]

[0060] Anti-interference agent:

[0061] Triton X-100 25g / L

[0062] Lauramidopropyl Betaine 15g / L

[0063] Reagent 2 (R2) is:

[0064]

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Abstract

A lipoprotein (a) determination kit comprises a reagent 1 and a reagent 2, the reagent 1 is composed of, by mass, a buffer solution, an inorganic salt, a surfactant, a stabilizer, a preservative and an anti-interference agent, and the reagent 2 is composed of, by mass, the buffer solution, the surfactant, the stabilizer, a sealing agent, lipoprotein (a) monoclonal antibody latex particles and the preservative. According to the kit disclosed by the invention, the anti-interference agent is added into the reagent 1, so that the anti-interference capability, the analysis sensitivity and the precision of the lipoprotein (a) latex immunoturbidimetry reagent are improved, the research and development cost of the reagent is not increased, and the kit is and the method are easier to adapt to the development of modern clinical laboratory medicine.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a lipoprotein (a) assay kit and a detection method thereof. Background technique [0002] Lipoprotein (a) [Lipoprotein (a), referred to as Lp (a)], is a special plasma lipoprotein rich in cholesterol, which was produced by Norwegian geneticist Berg in 1963 in the preparation of low-density lipoprotein (LDL) antibody A special macromolecular lipoprotein was first discovered during the process and was subsequently named lipoprotein (a). In the late 1980s, it was discovered that lipoprotein (a) was related to atherosclerosis. At the same time, Mclean found that the structure of lipoprotein (a) was highly homologous to plasminogen (PLG). [0003] The liver is the main place for the synthesis of lipoprotein (a), and its main physiological function is to prevent the dissolution of blood clots in blood vessels, and pathologically, it can promote atherosclerosis...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/92G01N33/577G01N33/543
CPCG01N33/92G01N33/577G01N33/54346G01N2333/775
Inventor 刘光华杨玉军
Owner 广州市伊川生物科技有限公司
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