A kind of remediation method of heavy metal polluted soil

A technology for polluted soil and restoration methods, which is applied in the restoration of polluted soil, land preparation methods, agricultural machinery and implements, etc. content, the probability of reducing the harm, the effect of efficient transformation effect

Active Publication Date: 2022-03-01
LANZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the treatment of heavy metal pollution in soil includes the soil replacement method, which can be effective immediately, but it cannot fundamentally solve the problem, and the project is huge; the thermal restoration method has high energy consumption, high cost, and is easy to damage the microbial structure of the soil; the current Bioremediation is a method recognized by the public, but its restoration of heavy metals in soil is relatively simple, often only effective for certain heavy metals, and most of the restoration effects are often less than 50%, especially for mercury and highly toxic heavy metals such as lead are difficult to handle

Method used

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  • A kind of remediation method of heavy metal polluted soil
  • A kind of remediation method of heavy metal polluted soil

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Preparation of immobilized microorganisms:

[0028] (1) after the palygorskite is pulverized to 90 orders, it is for subsequent use;

[0029] (2) After activation and purification of Pseudomonas on lysate broth (Luria-Bertani, LB) solid medium, inoculate in LB liquid medium and shake culture at 37°C and 150rpm until OD 600 0.7, then add 90-mesh palygorskite according to the dosage ratio of 1L:25g, shake and immobilize at a temperature of 37°C and a rotation speed of 100rpm for 12h, then filter, and freeze-dry at -20°C for 5h to obtain immobilized microorganisms;

[0030] Described LB liquid culture medium formula is:

[0031] Tryptone 10g, yeast extract 5g, NaCl 10g, deionized water 1000mL;

[0032] The LB solid medium formula is:

[0033] Tryptone 10g, yeast extract 5g, NaCl 10g, agar 17g, deionized water 1000 mL.

Embodiment 2

[0035] This embodiment provides a method for repairing heavy metal-contaminated soil, comprising the following steps:

[0036] S1, preparation of bioremediation agent: mix the immobilized microorganisms prepared in Example 1 with the lactic acid bacteria powder according to the mass ratio of 15:5 to obtain the bioremediation agent;

[0037] The brand name of the lactic acid bacteria powder is Xinlaiwang III-5000, including Lactobacillus acidophilus, Lactobacillus plantarum and Enterococcus faecium, the total number of lactic acid bacteria is ≥5×10 11 cfu / g, purchased from Xinlaiwang Biotechnology Co., Ltd.;

[0038] S2, add 90g of the bioremediation agent prepared in S1 to every 1 square meter of heavy metal-contaminated soil, plow the soil, and let it stand for 20 days;

[0039] S3, then add passivation agent to the soil after S2 remediation, the addition amount is 35g / m 3 , and then plow the soil again, and let it stand still for 25 days;

[0040] The passivating agent is...

Embodiment 3

[0045] This embodiment provides a method for repairing heavy metal-contaminated soil, comprising the following steps:

[0046] S1, preparation of bioremediation agent: mix the immobilized microorganisms prepared in Example 1 and lactic acid bacteria powder uniformly according to the mass ratio of 10:5 to obtain bioremediation agent;

[0047] The brand name of the lactic acid bacteria powder is Xinlaiwang III-5000, including Lactobacillus acidophilus, Lactobacillus plantarum and Enterococcus faecium, the total number of lactic acid bacteria is ≥5×10 11 cfu / g, purchased from Xinlaiwang Biotechnology Co., Ltd.;

[0048]S2, add 80g of the bioremediation agent prepared in S1 to every 1 square meter of heavy metal-contaminated soil, plow the soil, and let it stand for 10 days;

[0049] S3, then add passivation agent to the soil after S2 remediation, the addition amount is 30g / m 3 , and then plow the soil again, and let it stand still for 20 days;

[0050] The passivating agent is...

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Abstract

The invention relates to the field of soil remediation methods, and specifically discloses a method for remediation of heavy metal-contaminated soil. The invention uses a bioremediation agent to enrich and transform high-heavy metals in the soil to reduce the toxicity of heavy metals in the soil, and then uses a passivator to The heavy metals in the soil are passivated to generally reduce the toxicity of heavy metals. Finally, organic fertilizers are used to restore soil fertility, and organic fertilizers are used to further treat the heavy metals in the residual soil. The invention restores the soil polluted by heavy metals through progressive and step-by-step treatment, effectively reduces the content of toxic heavy metals in the soil, especially has an efficient conversion effect on mercury in the soil, and finally realizes the restoration of the soil.

Description

technical field [0001] The invention relates to the field of soil remediation methods, in particular to a method for remediation of heavy metal polluted soil. Background technique [0002] Heavy metal pollution refers to environmental pollution caused by metals or their compounds with a density above 5, mainly caused by human factors such as mining, exhaust gas discharge, sewage irrigation, and the use of heavy metal products. Due to the discharge of industrial "three wastes" motor vehicle exhaust, sewage irrigation and the use of pesticides, herbicides, fertilizers, etc., as well as the development of mining, the soil, water quality and atmosphere have been seriously polluted. In recent years, soil heavy metal pollution has attracted great attention. The basis for the development of high-quality agriculture is high-quality soil. With the development of science and technology, soil heavy metal pollution has become more and more serious. Heavy metals are more likely to endang...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B09C1/10B09C1/08A01B79/02
CPCB09C1/10B09C1/08A01B79/02B09C2101/00
Inventor 汪霞赵云飞郝志国王凯畅肖锦锦蒋思龙刘辉瑛周晓荷张富荣柳洋史常明
Owner LANZHOU UNIVERSITY
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