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Culicoides breeding method and culicoides culture device and application thereof

A breeding method and technology of Culicoides, applied in the fields of application, botanical equipment and methods, horticulture, etc., can solve the problems of difficulty in ensuring sufficient number of Culicoides with strong vitality at any time, not suitable for large-scale breeding, and many operations. , to shorten the research and development cycle, save manpower and time, and increase the number of eggs laid.

Active Publication Date: 2021-04-30
FOSHAN STANDARD BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The collection of Culicoides from the wild is limited by the season, environment, collection and transportation methods, and it is difficult to ensure a sufficient number of Culicoides with strong vitality for testing at any time
The currently reported method of cultivating Culicoides simply puts eggs or larvae in a container for cultivation, and needs to frequently change the water in the container to keep the water clean, and the device is simple and requires many operations, so it is not suitable for large-scale cultivation.

Method used

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  • Culicoides breeding method and culicoides culture device and application thereof
  • Culicoides breeding method and culicoides culture device and application thereof
  • Culicoides breeding method and culicoides culture device and application thereof

Examples

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Effect test

Embodiment 1

[0061] The cultivation method of the Culicoides of the present embodiment may further comprise the steps:

[0062] Incubation: place a layer of 80-mesh nylon cloth 310 on the stainless steel shelf 211 of the hatching basin 210 in the culture device, add water (the water added in the hatching basin is tap water that has been left standing for 2 days) until the water surface is flush with the stainless steel shelf 211, but not completely Pass nylon cloth 310, water depth 7cm, get 5g larvae food A and put it on nylon cloth 310, turn on the air pump, inject air into the water through air stone 212, form the microenvironment suitable for the life of Culicoid larvae in hatching basin 210 after 4 days, will contain The filter paper 1200 of Culicoides eggs is placed on the nylon cloth 310 in the hatching basin 210, the temperature is controlled at 28° C., and the humidity is 80%, for hatching and rearing to obtain larvae;

[0063] Larva rearing: After the larvae are observed, add larv...

Embodiment 2

[0067] The cultivation method of the Culicoides of the present embodiment may further comprise the steps:

[0068] Incubation: place a layer of 80-mesh nylon cloth 310 on the stainless steel shelf 211 of the hatching basin 210 in the culture device, add water (the water added in the hatching basin is tap water that has been left standing for 2 days) until the water surface is flush with the stainless steel shelf 211, but not completely Pass nylon cloth 310, water depth 7cm, get 5g larvae food A and put it on nylon cloth 310, turn on the air pump, inject air into the water through air stone 212, form the microenvironment suitable for the life of Culicoid larvae in hatching basin 210 after 4 days, will contain The filter paper 1200 of Culicoides eggs is placed on the nylon cloth 310 in the hatching basin 210, the temperature is controlled at 28° C., and the humidity is 80%, for hatching and rearing to obtain larvae;

[0069] Larva rearing: After the larvae are observed, add larv...

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Abstract

The invention belongs to the technical field of insect breeding, and discloses a culicoides breeding method and a culicoides breeding device and application. The culicoides breeding method comprises the following steps that larva foodstuff A is put in the breeding device, water and air are introduced, then culicoides eggs are put, and hatching is carried out to obtain larvae; after larvae are obtained, the temperature and humidity are controlled, the larva foodstuff A and larva foodstuff B are put, and the larvae are pupated; and green plants and an adult nutrition bag are placed in a breeding cage of the breeding device, and pupae are subjected to eclosion to form culicoides; blood is fed to the culicoides, eggs are laid, the culicoides is taken out, and next-generation incubation is carried out. The larva foodstuff A can be used as a larva foodstuff and can be used for larvae to adhere and pupate, animal blood is added to the larva foodstuff B on the basis of the larva foodstuff A, nutrition can be supplemented, and the number of the laid next generation of the eggs is obviously increased.

Description

technical field [0001] The invention belongs to the technical field of insect breeding, and in particular relates to a breeding method for Culicoides, a breeding device and an application thereof. Background technique [0002] Culicoides are blood-sucking insects belonging to the order Diptera, the family Ceratopogonidae, and the genus Culicoides. They are small in size, with large eyes, short beaks, and slender antennae. Culicoides usually live in a warm and humid environment, and high temperature, dryness and cold are not conducive to the development of Culicoides. Therefore, a large number of Culicoides can be collected during rainy spring and summer, but only a small number of Culicoides can be seen in autumn and winter. The life cycle of Culicoides generally lasts 3 to 6 weeks, including four stages: egg, larva, pupa, and adult. Some species of female midges need to suck the blood of animals 3 to 4 days before laying eggs, while male midges feed on plant juices. [0...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033A23K50/90A23K20/163A23K10/16A23K10/24A23K10/30A23K10/20A01G22/40A01G22/15A01G22/00
CPCA01K67/033A23K50/90A23K20/163A23K10/16A23K10/24A23K10/30A23K10/20A01G22/40A01G22/15A01G22/00
Inventor 邝春曼谭志坚王新秋刘丽丹翁亚彪黄仪娟曾莉刘园
Owner FOSHAN STANDARD BIO TECH
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