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Extract with nematode inhibiting effect, streptomyces and applications of extract and streptomyces

A technology of extract, Streptomyces grisea, applied in applications, nematocides, chemicals for biological control, etc., can solve the problems of photosynthesis decline, water and nutrient transportation obstruction, yield decline, etc., and achieve high root resistance The effect of nematode activity

Active Publication Date: 2021-04-27
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Root-knot nematodes destroy the normal differentiation and physiological activities of root tissue, impede the transportation of water and nutrients, reduce photosynthesis, and often produce deformed or unevenly colored fruits, resulting in reduced yield and quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Embodiment 1, isolation, identification and preservation of bacterial strains

[0048] 1. Collection of strain MS180023

[0049] In August 2018, a strain was isolated from sea mud in the South China Sea, named strain MS180023.

[0050] 2. Identification of strain MS180023

[0051] Morphological characteristics: the colony is raised, the mycelium grows vigorously, the mycelium in the base is light yellow, the aerial mycelium appears white, and there are white spores, and no water-soluble pigment is produced.

[0052] The partial sequence of the 16S rRNA gene is shown in the sequence 1 of the sequence list, and has the highest sequence similarity with GENBANK ACCESSION NO.KY783422.1, with a similarity of 99.93%.

[0053] According to the identification results, the strain MS180023 belongs to Streptomyces griseorubens.

[0054] 3. Preservation of strain MS180023

[0055] Streptomyces griseorubens MS180023 was deposited in the General Microorganism Center of China Committ...

Embodiment 2

[0056] Example 2. Preparation of crude extract of Streptomyces grisea MS180023 and detection of its inhibitory effect on root-knot nematode 1. Preparation of crude extract of Streptomyces grisea MS180023

[0057] 1. Streptomyces grisea MS180023 was streak-inoculated on the slant medium, and cultured at 28° C. for 5 days (to make the aerial hyphae grow plumply) to obtain the slant strain.

[0058] Preparation method of slant medium: dissolve 4 grams of yeast extract, 10 grams of malt extract, 4 grams of glucose and 20 grams of agar powder in water, adjust the pH to 7.0-7.2, dilute to 1L with water, and extinguish at 115 °C Bacteria for 30 minutes.

[0059] 2. Dispense the seed culture medium into 500ml Erlenmeyer flasks (100ml / bottle), inoculate the slanted bacterial species into the seed culture medium, shake and culture at 28°C and 200rpm for 5 days to obtain OD 600nm = 1.2-1.4 seed liquid.

[0060] The preparation method of the seed medium: dissolve 4 grams of yeast extrac...

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Abstract

The invention discloses an extract with a nematode inhibiting effect, streptomyces and applications of the extract and the streptomyces. The invention provides a method for preparing a streptomyces griseorubens fermentation product. The method comprises the following steps: fermenting streptomyces griseorubens MS180023 to obtain a fermentation product. The invention also provides a method for preparing the streptomyces griseorubens extract. The method comprises the following steps: taking a supernatant of the fermentation product, performing extracting with an organic reagent, and collecting an extracting solution; and carrying out reduced pressure distillation on the extracting solution to remove an organic solvent so as to obtain the extract. The invention also protects the streptomyces griseorubens MS180023. The invention also protects the applications of the streptomyces griseorubens MS180023, the fermentation product, the supernatant of the fermentation product or the extract: the application of the streptomyces griseorubens MS180023 in preparation of a nematode inhibitor; and (b) the application of the compound in inhibition of nematodes.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an extract capable of inhibiting nematodes, streptomycetes and their applications. Background technique [0002] Root-knot nematodes are a class of plant parasitic nematodes that cause plant roots to form root knots, making plants more susceptible to other fungal and bacterial diseases. Nematodes can infest more than 2,000 plant species. [0003] Root-knot nematode mainly lives in the soil with eggs and 2nd instar larvae along with root nodules, or overwinters directly in the soil, and can survive for 3 years without a host plant in the soil. The 2nd instar larvae are the infection stage of root-knot nematodes and usually invade from the root tip. The nematodes grow and develop to the 4th instar in the root knot or root nodule of the host, the male and the female mate, and the female lays eggs in the root knot after mating, and the male worm drills out of the host tissu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04A01N63/28A01P5/00C12R1/465
CPCC12P1/04A01N63/28
Inventor 宋福行杨娜王龙
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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