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Preparation and application of electrochemiluminescence sensor for detecting I-type procollagen amino-terminal protopeptide iridium nanocrystals

A technology of primary amino and nanocrystals, applied in the field of nanomaterials, can solve the problems of easy agglomeration, poor water solubility, and limited applications

Active Publication Date: 2021-04-20
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Ir(mppy) 3 Poor water solubility, easy aggregation, and poor biocompatibility greatly limit its application in aqueous immunoassays

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1. Preparation and application of an iridium nanocrystal electrochemiluminescence sensor for detecting type I procollagen N-terminal propeptide, the steps of the iridium nanocrystal solution are as follows:

[0034] At room temperature, 10 mg of tris[2-(p-tolyl)pyridine-C2,N]iridium Ir(mppy) 3 The solid was dissolved in 1 mL of tetrahydrofuran to prepare Ir(mppy) with a mass concentration of 10 mg / mL 3 solution, measure 0.5 mL of Ir(mppy) with a mass concentration of 10 mg / mL 3 The solution was added dropwise to 8.4 mL bovine serum albumin (BSA) solution with a mass concentration of 5 mg / mL, at 55 o After stirring continuously for 2 h at C, then add 0.1 mL of alkali solution with a molar concentration of 0.1 mol / L to adjust the solution to pH 12, at 37 o Stirring was continued at C for 12 h to obtain a dark yellow precipitate. The product was separated and purified by ultrafiltration and centrifugation, and the final iridium nanocrystals were dispersed into...

Embodiment 2

[0035] Example 2. Preparation and application of an iridium nanocrystal electrochemiluminescent sensor for detecting type I procollagen N-terminal propeptide. The preparation steps of the iridium nanocrystal solution are as follows:

[0036] At room temperature, 15 mg of tris[2-(p-tolyl)pyridine-C2, N]iridium Ir(mppy) 3 The solid was dissolved in 2 mL of tetrahydrofuran to prepare Ir(mppy) with a mass concentration of 7.5 mg / mL 3 solution, measure 1 mL of Ir(mppy) with a mass concentration of 7.5 mg / mL 3 The solution was added dropwise to 8.9 mL of bovine serum albumin BSA solution with a mass concentration of 10 mg / mL, at 55 o After stirring continuously for 3 h at C, then add 0.1 mL of alkali solution with a molar concentration of 0.1 mol / L to adjust the solution to pH 12, at 37 o Stirring was continued at C for 18 h to obtain a dark yellow precipitate. The product was separated and purified by ultrafiltration and centrifugation, and the final iridium nanocrystals were dis...

Embodiment 3

[0037] Example 3. Preparation and application of an iridium nanocrystal electrochemiluminescence sensor for detecting type I procollagen N-terminal propeptide. The preparation steps of the iridium nanocrystal solution are as follows:

[0038] At room temperature, 20 mg of tris[2-(p-tolyl)pyridine-C2,N]iridium Ir(mppy) 3The solid was dissolved in 4 mL of tetrahydrofuran to prepare Ir(mppy) with a mass concentration of 5 mg / mL 3 solution, measure 1.5 mL of Ir(mppy) with a mass concentration of 5 mg / mL 3 The solution was added dropwise to 8.4 mL of bovine serum albumin BSA solution with a mass concentration of 10 mg / mL, at 55 o After stirring continuously for 4 h at C, then add 0.1 mL of alkali solution with a molar concentration of 0.1 mol / L to adjust the solution to pH 12. o Stirring was continued at C for 24 h to obtain a dark yellow precipitate. The product was separated and purified by ultrafiltration and centrifugation, and the final iridium nanocrystals were dispersed in...

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PUM

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Abstract

The invention relates to preparation and application of an electrochemiluminescence sensor for detecting I-type procollagen amino-terminal protopeptide iridium nanocrystals, and is characterized in that the iridium nanocrystals with good water solubility, good biocompatibility and high electrochemiluminescence efficiency are prepared, an antibody-labeled iridium nanocrystal solution is used as a signal probe to develop a label-free electrochemiluminescence immunosensor, the sensor is used for rapid and sensitive detection of multiple myeloma marker I-type procollagen amino terminal propeptide, the detection limit is 35 fg / mL, the linear range is 100 fg / mL and 50 ng / mL, and the sensor has the advantages of being high in specificity, good in reproducibility, stable in signal and the like.

Description

technical field [0001] The invention belongs to the field of nanometer materials and the field of biosensing technology. Background technique [0002] As a research hotspot arising from the intersection of biology, chemistry, medicine, electronic technology and other disciplines, electrochemiluminescence immunoassay is an organic combination of electrochemiluminescence technology and immunoassay method, and the prepared immunosensor has low cost , good selectivity, high sensitivity, fast analysis speed, easy automation, miniaturization and integration, etc., have been widely used in disease marker analysis, food safety analysis, environmental pollution analysis and other fields. [0003] Multiple myeloma is a malignancy of clonal proliferation of plasma cells that causes bone damage, often accompanied by bone disease. It can be seen that it is very important to find a serum non-invasive biological index that can be used for multiple myeloma diagnosis and condition monitorin...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327G01N21/76G01N33/53G01N33/531G01N33/543G01N33/574B82Y15/00
Inventor 杜宇任祥刘蕾刘雪静张诺魏琴鞠熀先
Owner UNIV OF JINAN
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