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Application of macrophage subsets and their regulators in acute graft-versus-host disease

A graft-versus-host, macrophage technology, applied in the field of biomedicine

Active Publication Date: 2022-02-01
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report to improve the normalization of the microenvironment of peripheral T cells in aGVHD by regulating the expression of transcription factor KLF4 to regulate the polarization of macrophages, and ultimately improve the function of T cells

Method used

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  • Application of macrophage subsets and their regulators in acute graft-versus-host disease
  • Application of macrophage subsets and their regulators in acute graft-versus-host disease
  • Application of macrophage subsets and their regulators in acute graft-versus-host disease

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Example 1. Application of different macrophage subpopulations and their regulators in acute graft-versus-host disease

[0047] 1. Experimental materials

[0048] 1. Monoclonal antibody: CD68-FITC (BD Biosciences), CCR2-Alexa Fluor 647 (BD Biosciences), CX3CR1-PerCP / Cy5.5 (Biolegend)), CD163-PE / Cy7 (Biolegend), CD14-PE (Biolegend) , CD16-APC / Cy7 (Biolegend), IL-6-APC (BD Biosciences), TGF-β-BV421 (BD Biosciences), TNF-ɑ-BV650 (BD Biosciences). CD3-APC / H7 (BD Biosciences), CD8-BV510 (Biolegend), CD25-PE / Cy7 (Biolegend), IFN-γ-PerCP / Cy5.5 (Biolegend), IL-4-PE (BD Biosciences), IL17A- FITC (BD Biosciences), FoxP3-eFlour660 (eBioscience).

[0049] 2. DAPI solution: Item No. C0065, Beijing Suolaibao Company.

[0050] 3. Diacetylated low-density lipoprotein: DiI-AcLDL, Life Technologies.

[0051] 4. Recipient BALB / c mice and donor C57BL / 6 mice (Beijing Weitong Lihua Experimental Animal Technology Co., Ltd.).

[0052] 5. Recombinant human M-CSF: Catalog#300-25, American PE...

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Abstract

The invention discloses the application of macrophage subpopulation and its regulator in acute graft-versus-host disease. The present invention protects the following applications of substances for detecting the expression of KLF4 transcription factor or its encoding nucleic acid in macrophages: preparing and detecting the polarization / functional state of macrophages in patients with aGVHD, diagnosing aGVHD, and / or detecting whether M1 / function occurs in macrophages The product of M2 polarization imbalance. The present invention found that a significant decrease in KLF4 in macrophages is a biomarker of functionally altered M1 / M2 polarization imbalance. The invention can be used to detect the polarization and functional state of macrophages in patients with aGVHD after hematopoietic stem cell transplantation, and has important clinical significance for stratified diagnosis and treatment of diseases.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of a macrophage subgroup and its regulator in acute graft-versus-host disease. Background technique [0002] Acute graft-versus-host disease (aGVHD) remains an important complication of allogeneic hematopoietic stem cell transplantation. aGVHD is generally regarded as an immune-mediated disease, specifically, the T lymphocytes in the allogeneic donor graft are stimulated by a series of "cytokine storms" launched by the recipient, which greatly enhances their response to recipient antigens. The immune response launches a cytotoxic attack targeting the target cells of the recipient, among which the skin, liver and intestinal tract are the main targets, resulting in increased patient-related mortality and medical costs. Glucocorticoids are currently the first-line treatment for aGVHD, but 40-60% of patients still develop steroid resistance, and the current second-line tre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/00
Inventor 黄晓军孔圆温琦赵红艳
Owner PEOPLES HOSPITAL PEKING UNIV
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