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Application of morpholine in controlling unsaponifiable matter content in schizochytrium limacinum fermentation process and increasing DHA grease content

A technology for the fermentation process of Schizochytrium, which is applied in the field of microorganisms, can solve the problems that the sterol and oil content of Schizochytrium strains have not been reported, and achieve the effects of promoting growth and DHA accumulation, and reducing the sterol content

Active Publication Date: 2021-04-16
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is no report on the control of sterol and oil content of Schizochytrium strains by adding morpholine exogenously

Method used

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  • Application of morpholine in controlling unsaponifiable matter content in schizochytrium limacinum fermentation process and increasing DHA grease content
  • Application of morpholine in controlling unsaponifiable matter content in schizochytrium limacinum fermentation process and increasing DHA grease content
  • Application of morpholine in controlling unsaponifiable matter content in schizochytrium limacinum fermentation process and increasing DHA grease content

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Seed medium: glucose 30.0g / L, yeast powder 10.0g / L, sodium sulfate 12.0g / L, calcium chloride dihydrate 0.17g / L, potassium chloride 0.50g / L, magnesium sulfate 2.0g / L, sulfuric acid Potassium 0.65g / L, potassium dihydrogen phosphate 1.0g / L;

[0039] Fermentation medium: glucose 80.0g / L, sodium glutamate 5.0g / L, yeast powder 5.0g / L, sodium sulfate 12.0g / L, potassium chloride 0.50g / L, magnesium sulfate 2.0g / L, potassium sulfate 0.65g / L, potassium dihydrogen phosphate 1.0g / L, calcium chloride dihydrate 0.17g / L, ammonium sulfate 1.0g / L

[0040] Concentration of added morpholine: 1.0g / L, 1.1g / L, 1.2g / L, 1.3g / L, 1.4g / L, 1.5g / L, 1.6g / L, 1.7g / L, 1.8g / L , 1.9g / L, 2.0g / L, 2.1g / L, 2.2g / L, 2.3g / L, 2.4g / L, 2.5g / L. During the experiment, the composition and addition amount of the medium remained unchanged, only morpholine was added to different fermentation medium, and a control experiment was done.

[0041] The method for controlling the content of unsaponifiable matter in the ferme...

Embodiment 2

[0061] Fed-batch Fermentation Cultivation of Schizochytrium Strain in 5L Fermenter

[0062] The seed liquid (secondary seed liquid among the embodiment 1) of the preserved Schizochytrium is inserted in the 5L fermenter that sterilized 3L fermentation medium is housed with the inoculum size of 1% (v / v) , fermented to 12h culture medium and added the morpholine of 1.8g / L (other formulations are the same as embodiment 1), at 28 ℃, the aeration rate of 1vvm, the stirring speed of 170rpm under fermentation culture 48h, extract fermented liquid and measure each index content. The results are shown in Table 2.

[0063] Table 2 Comparison of fermentation results before and after adding 1.8g / L morpholine

[0064]

[0065]

[0066] In the final 5L fermenter, add 1.8g / L morpholine, and the final result obtained by fermentation oil production is compared with the original strain, the content of sterols is significantly decreased, and the content of astaxanthin is significantly incr...

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Abstract

The invention discloses application of morpholine in controlling unsaponifiable matter content and increasing DHA grease content in a schizochytrium limacinum fermentation process, and aims to reduce sterol content and increase grease content in the schizochytrium limacinum fermentation process by exogenously adding morpholine in the schizochytrium limacinum fermentation process. In addition, optimum addition concentration of morpholine is determined according to the biomass of cell growth of schizochytrium limacinum, the oil yield and the unsaponifiable matter content index; compared with the situation that 1.8 g / L of morpholine is added, the sterol content is reduced by 64.86%, the oil yield is increased by 67.51%, the DHA content is increased by 25.5%, and the astaxanthin content is increased by 85%. By means of the technical scheme, the content of DHA grease and astaxanthin produced by the schizochytrium limacinum is obviously improved. In the invention, the morpholine is applied to regulation and control of the unsaponifiable matter content of schizochytrium limacinum for the first time, wherein a new thought is provided for increasing the content of grease in schizochytrium limacinum, and the method has huge potential in the aspect of industrial production.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to the application of morpholine in controlling the content of unsaponifiable matter in the fermentation process of Schizochytrium, increasing the content of DHA oil and astaxanthin, and a specific method thereof. Background technique [0002] Docosahexaenoic acid (DHA) is an important omega-3 series polyunsaturated fatty acid, which has many important physiological effects on human health. The raw materials for traditional DHA production mainly come from deep-sea fish oil, but the shortage of fish oil resources, the unique fishy smell of fish oil, poor oxidation stability, susceptibility to marine chemical pollution and high processing costs have caused the oil content and unsaturated fatty acids in the oil to be reduced. Content instability, and low yield. Therefore, finding a new source of DHA has become the research focus of single-cell lipids. [0003] Accor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/64C12P23/00C12R1/645
Inventor 黄和杜菲孙小曼许莹霜王伶如
Owner NANJING NORMAL UNIVERSITY
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