Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of FICZ in inhibition of tumor cell migration

A glioma cell, cell technology, applied in the application of FICZ in inhibiting the migration of glioblastoma cells, in the field of cancer treatment, can solve the problems of poor prognosis and low long-term survival rate

Inactive Publication Date: 2021-04-16
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Comprehensive treatment of glioblastoma includes surgery, radiotherapy, systemic therapy (chemotherapy, targeted therapy) and supportive care, etc., but the overall prognosis is still poor, long-term low survival rate

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of FICZ in inhibition of tumor cell migration
  • Application of FICZ in inhibition of tumor cell migration
  • Application of FICZ in inhibition of tumor cell migration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1FI

[0053] Example 1 Exploration of the Toxic Effect of FICZ on Glioblastoma

[0054] U87 cells were treated with 1×10 5 Cells / mL were inoculated into 96-well plates with 100 mL of cell suspension per well. Incubate overnight to allow cells to attach. Take out the 96-well plate, aspirate the original medium, and wash once with phosphate buffered saline (PBS). The experiment set up background control group, solvent control group and drug treatment group. Correspondingly, low-serum medium (1% serum concentration, no cells in the background control well), low-serum medium containing solvent dimethyl sulfoxide (DMSO) or different concentrations of FICZ was added to each well, and the volume of the solvent was maintained at 0.1%. Put the 96-well plate after adding the drug into the incubator to continue culturing for 24h or 48h. After reaching the end point of the culture, add 10 μL of CCK8 reagent to each well according to the instructions of the kit, put the well plate into the ...

Embodiment 2FI

[0056] Example 2FICZ inhibits the migration of glioblastoma cells

[0057] Cell migration ability was evaluated by scratch test. Use a marker pen to draw 3 straight lines horizontally on the bottom of the 6-well plate to locate the scratches. cells in 4 x 10 5 Inoculate 2 mL of cells / mL in a 6-well plate, culture overnight and wait for the cells to adhere to the wall. After the cells have completely covered the bottom of the well plate, scratch the cell monolayer with the tip of a 1 mL pipette to form a scratch. Choose the junction of the scratch and the marker line as the photo location. Floating cells were removed by washing with phosphate-buffered saline, and then low-serum medium containing the solvent DMSO or FICZ was added. Images were collected under an inverted microscope with a digital camera at 0-24 hours after scratching. A total of three independent repeated experiments were carried out, each concentration group in each independent experiment had 3 replicate w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to application of FICZ in inhibition of cell migration, in particular to application of FICZ or a pharmaceutical composition containing FICZ in preparation of a drug for inhibiting glioma cell migration. It is found for the first time that FICZ with a specific concentration can inhibit migration of a glioblastoma cell line U87, and a new choice is provided for treatment of glioblastoma.

Description

technical field [0001] The present invention relates to the field of molecular biology, more specifically, the present invention relates to technologies related to cancer treatment. The invention mainly includes the application of FICZ (6-methoxyindole [3,2-b]carbazole) in inhibiting tumor cell migration, and particularly discloses the application of FICZ in inhibiting glioblastoma cell migration. Background technique [0002] Brain and central nervous system tumors are the third leading cause of cancer-related death in all cancer-related case studies. Among brain tumors, astrocytoma has a high incidence, accounting for 15.1% of primary central nervous system tumors and 46.1% of primary central nervous system malignant tumors. Among them, glioblastoma is the most common, which belongs to a highly aggressive primary glioma, and is also a grade IV tumor in the World Health Organization (WHO) tumor malignancy classification. [0003] The comprehensive treatment of glioblastom...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/404A61P35/04A61P25/00
Inventor 赵斌刘奕耘谢群慧徐丽陈旸升
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com