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A kind of method that takes water hyacinth as raw material to prepare lactic acid

A water hyacinth and lactic acid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of preparing lactic acid, etc., achieve the effects of rich nutrition, protection of the environment and ecological balance, and avoiding waste

Active Publication Date: 2022-03-08
ANHUI BBCA FERMENTATION TECH ENG RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There is no technology to produce lactic acid through the action of microorganisms using water hyacinth, a fibrous plant

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] This embodiment provides a method for preparing lactic acid from water hyacinth as a raw material, comprising the following steps:

[0037] (1) Collect 100kg of fresh water hyacinth and wash and chop it, use a pulverizer to pulverize, then use a centrifuge to separate the solid from the liquid, and collect the solid and clear liquid respectively;

[0038] (2) Alkali treatment of raw materials: add the solid matter and 0.7% NaOH solution obtained in step (1) into the reactor according to the solid-to-liquid ratio of 1:20, mix well, and maintain the temperature at 120°C for 60 minutes, then Lower the temperature to 40°C, use a centrifuge to separate the solid and liquid from the obtained mixture, and collect the solid and clear liquid separately;

[0039] (3) Add protease to the fermenter for hydrolysis: add the solid matter obtained in step (2) and the clear liquid obtained in step (1) into the fermenter according to the solid-to-liquid ratio of 3:10, mix well, and use H...

Embodiment 2

[0046] This embodiment provides a method for preparing lactic acid from water hyacinth as a raw material, comprising the following steps:

[0047] (1) Collect 150 kg of fresh water hyacinth and wash and chop it, use a pulverizer to pulverize, then use a centrifuge to separate the solid from the liquid, and collect the solid and clear liquid respectively;

[0048] (2) Alkali treatment of raw materials: add the solid matter obtained in step (1) and 0.9% NaOH solution into the reactor according to the solid-to-liquid ratio of 1:22, mix well, and maintain the temperature at 120°C for 80 minutes, then Lower the temperature to 42°C, use a centrifuge to separate the solid and liquid from the obtained mixture, and collect the solid and clear liquid separately;

[0049] (3) Add protease to the fermenter for hydrolysis: add the solid matter obtained in step (2) and the clear liquid obtained in step (1) into the fermenter according to the solid-to-liquid ratio of 1:2.85, mix well, and use ...

Embodiment 3

[0056] This embodiment provides a method for preparing lactic acid from water hyacinth as a raw material, comprising the following steps:

[0057] (1) Collect 50 kg of fresh water hyacinth and wash and chop it, use a pulverizer to pulverize, then use a centrifuge to separate the solid from the liquid, and collect the solid and clear liquid respectively;

[0058] (2) Alkali treatment of raw materials: Add the solid matter and 0.5% NaOH solution obtained in step (1) into the reactor according to the solid-to-liquid ratio of 1:18, mix well, and maintain the temperature at 120°C for 40 minutes, then Lower the temperature to 39°C, use a centrifuge to separate the solid and liquid from the obtained mixture, and collect the solid and clear liquid separately;

[0059] (3) Add protease to the fermenter for hydrolysis: add the solid matter obtained in step (2) and the clear liquid obtained in step (1) into the fermenter according to the solid-to-liquid ratio of 1:3.85, mix well, and use...

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Abstract

The invention discloses a method for preparing lactic acid using water hyacinth as a raw material. The method comprises the following steps: crushing and centrifuging water hyacinth, treating the solid matter with alkaline solution, adding protease for hydrolysis, sterilization, and adding cellulase for lactic acid preparation. Saccharification, adding lactic acid bacteria for fermentation to obtain lactic acid. The method of the present invention utilizes water hyacinth, an invasive species, as a raw material, and generates lactic acid by treating it with microorganisms, which not only rationally utilizes this abundant resource, reduces the harm caused by water hyacinth, but also further improves the waste water produced in the production process. Reasonable utilization, saving water resources, playing the role of turning waste into wealth, protecting the ecological environment, saving resources, and at the same time providing new raw materials for the production of lactic acid. The present invention has important significance in the field of production of lactic acid.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a method for preparing lactic acid using water hyacinth as a raw material. Background technique [0002] The Chinese name of water hyacinth is water hyacinth, the Latin name is Eichhornia crassipes (Mart.) Solms, and the English name is water hyacinth. Water hyacinth is native to South America and can grow in an environment of 5-39°C. Its growth and reproduction speed is extremely fast, and the number of plants can double in 5 days. my country introduced water hyacinth in the 1930s, mainly used to deal with water pollution, and also used as feed, fertilizer and fuel. In recent years, due to the serious eutrophication of water bodies, water hyacinth has grown and flooded in South China, East China, Central China and North China, and is regarded as a harmful organism that pollutes the environment, causing serious environmental and social problems, inclu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C12R1/01
CPCC12P7/56
Inventor 穆晓玲常乐赵亚飞王舒孟瑶曹曙光
Owner ANHUI BBCA FERMENTATION TECH ENG RES
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