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Set of SNP molecular markers for detecting purity of sunflower variety and use of set of SNP molecular markers

A molecular marker, variety purity technology, applied in the field of agricultural molecular biology

Active Publication Date: 2021-04-09
HUAZHI RICE BIO TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the SSR marker method is mainly used for the detection of sunflower purity; there are few reports on the detection method of sunflower purity based on SNP markers

Method used

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  • Set of SNP molecular markers for detecting purity of sunflower variety and use of set of SNP molecular markers
  • Set of SNP molecular markers for detecting purity of sunflower variety and use of set of SNP molecular markers
  • Set of SNP molecular markers for detecting purity of sunflower variety and use of set of SNP molecular markers

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Embodiment Construction

[0051] In order to describe the technical content, achieved goals and effects of the present invention in detail, the following descriptions will be made in conjunction with the embodiments and accompanying drawings. The test methods used in the examples are conventional methods unless otherwise specified; the used materials, reagents, etc., are commercially available reagents and materials unless otherwise specified.

[0052] In order to describe the technical content, achieved goals and effects of the present invention in detail, the following descriptions will be made in conjunction with the embodiments and accompanying drawings. The test methods used in the examples are conventional methods unless otherwise specified; the used materials, reagents, etc., are commercially available reagents and materials unless otherwise specified.

[0053] Embodiments of the present invention are: SNP molecular markers for sunflower variety purity detection

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Abstract

The invention discloses a set of SNP molecular markers for detectingpurity of sunflower variety and use of set of SNP molecular markers. The set of 17 SNP molecular markers can be used for detecting purities of oil sunflower and edible sunflower varieties and genetic materials with different sources, have wide use universality, and can trace and speculate a pollution source of impure samples. The set of SNP molecular markers are co-dominant, and have high specificity, sensitivity and resolution. The markers are not influenced by environmental conditions, seeds or any type of plant tissues can be used, and detection results are accurate, repeatable and stable. The technology is simple and easy for automation, and has high detection flux and high speed. Unit data point detection cost is low. Data results of different detection laboratories can be compared and verified with each other, and the data has universal comparability.

Description

technical field [0001] The invention belongs to the field of agricultural molecular biology, and in particular relates to a set of SNP molecular markers for detecting the purity of sunflower varieties and applications thereof. Background technique [0002] Sunflower (Helianthus annuus L.) is divided into three categories: oil sunflower (mainly used for oil extraction), food sunflower (casual snacks and desserts) and ornamental sunflower. Sunflower is the fourth largest oil-bearing crop next to soybeans, rapeseed, and peanuts; oil sunflowers are the main sunflower-producing countries in Europe and the United States such as Ukraine, Russia, the European Union, and Argentina, accounting for more than 90% of the total sunflower cultivation area. Since the 1990s, my country's sunflower breeding and production have made great progress. At present, China is the largest producer of edible sunflower in the world. About 50% of the area. Sunflower has become an important regional char...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11A01H1/04
CPCC12Q1/6895A01H1/04C12Q2600/156
Inventor 唐顺学李乐彭佩肖金华田冰川
Owner HUAZHI RICE BIO TECH CO LTD
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