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SCAR marker for identifying Tremella aurantialba X9 strain or Tremella aurantialba strain containing X9 strain

A technique for identifying Aureus and Aureus, applied in DNA/RNA fragments, recombinant DNA technology, microbe determination/inspection, etc.

Pending Publication Date: 2021-04-09
云南菌视界生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The existing reports on the research on Auricularia species all involve conventional methods of strain propagation, and the description of the quality of the strains only involves the difference between effective strains and invalid strains [6-8] , the identification of Auricularia species and the development of molecular markers have not been reported, which belongs to the blank field

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  • SCAR marker for identifying Tremella aurantialba X9 strain or Tremella aurantialba strain containing X9 strain
  • SCAR marker for identifying Tremella aurantialba X9 strain or Tremella aurantialba strain containing X9 strain
  • SCAR marker for identifying Tremella aurantialba X9 strain or Tremella aurantialba strain containing X9 strain

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Embodiment

[0057] 1. Collection of Auricularia strains

[0058] Fresh fruiting bodies of 7 different origins of Auricularia auricula were collected from the market by visiting the growers of Auricularia aurantiaceae and the fresh Auricularia auritii mushroom trading market, and the strains were obtained after separation and purification by tissue separation method, and the numbers corresponding to the origins were: NL( Ninglang), YL (Yunlong), YR (Yongren), CX (Chuxiong), QJ (Qujing), ZY (Zhangye), SC (Sichuan); in addition, the inventor independently cultivated 4 containing Auricularia X9 Golden ear strains of strains: JSJ-X9, JSJ-X18, JSJ-H1, JSJ-H3. A total of 11 golden ear strains were collected.

[0059] 2. Acquisition of Aureus strains (asexual spores)

[0060] Auricularia X9 strain is used for direct propagation, and the fruiting bodies of 11 Auricularia strains are surface-sterilized, and 4-6 pieces of fruiting bodies of each Auricularia strain are taken 5×5×5mm 3 The size of ...

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Abstract

The invention belongs to the field of edible fungus cultivation, and particularly relates to a SCAR marker for identifying a Tremella aurantialba X9 strain or a Tremella aurantialba strain containing X9 strain. For Tremella aurantialba strains, the SCAR marker is used for identification, so that the Tremella aurantialba X9 strains can be distinguished from other Tremella aurantialba strains; for Tremella aurantialba, asexual spores, basidiospores, and basidiospore cultures (spores) of Tremella aurantialba strains are manipulated when identified with SCAR markers, such that Tremella aurantialba strains containing X9 strains can be distinguished from other Tremella aurantialba strains. The SCAR marker has high characteristics in a Tremella aurantialba X9 strain and a Tremella aurantialba strain containing the X9 strain, and the detection result is stable and reliable.

Description

technical field [0001] The invention belongs to the field of edible fungus cultivation, and in particular relates to a SCAR marker for identifying the X9 strain of Auricularia aureus or the Auricularia aureus strain containing the X9 strain. [0002] technical background [0003] SCAR (sequence characterized amplified regions, sequence-specific amplified regions) markers are usually converted from RAPD, SRAP, and SSR markers. SCAR labeling is to recover the characteristic marker fragments from the gel, clone and sequence them, and design a pair of characteristic primers (18-24 bases) according to their base sequences; SCAR markers generally show the presence or absence of amplified fragments, which can be used for Rapidly detect large numbers of individuals. The primers used for SCAR labeling are long and the primer sequence is completely complementary to the template DNA, which can be amplified under stringent conditions, so the results are stable and reproducible. Due to ...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12Q1/04C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/156C12Q2531/113C12Q2565/125
Inventor 李荣春杨林雷
Owner 云南菌视界生物科技有限公司
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