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Human esophageal squamous cell carcinoma circular RNA marker and application thereof

A technology for squamous cell carcinoma of the esophagus, applied in the field of biomedicine, can solve difficult problems and achieve the effects of improving clinical prognosis, not easy to degrade, and improving accuracy

Pending Publication Date: 2021-04-09
THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is of great significance to construct a prognostic lncRNA or circular RNA marker with high potential clinical application value, but it is still difficult to find a single lncRNA or circular RNA marker with high sensitivity and specificity in ESCC prognosis prediction in clinical practice

Method used

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  • Human esophageal squamous cell carcinoma circular RNA marker and application thereof
  • Human esophageal squamous cell carcinoma circular RNA marker and application thereof
  • Human esophageal squamous cell carcinoma circular RNA marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Determining the expression characteristics of circular RNAs associated with the prognosis of ESCC patients.

[0042]Based on 73 pairs of ESCC patient RNA-seq (ribosome sequencing) data circular RNA expression profiles and clinical follow-up data, we screened out 1202 prognostic-related circular RNAs, of which 1029 circular RNAs were derived from exons (exon ), 105 circular RNAs were derived from intergenic regions, and 68 circular RNAs were derived from introns (Intron) (see figure 1 ).

[0043] In order to obtain the combination with the best prognostic ability, the applicant conducted a random survival forest variable analysis (RSFVH analysis) for the top 300 and 200 highly expressed circular RNAs among the 1202 prognostic-related circular RNAs, and screened out 17 For prognostic-related circular RNAs, the 17 circular RNAs were verified in ESCC tissues and cell lines by designing primers, and only 9 circular RNAs were found to be expressed, and the other 8...

Embodiment 2

[0047] Example 2: Expression of 4 circular RNA markers in 8 randomly selected ESCC patient cancer tissues and cell lines.

[0048] It was found that 4 circular RNAs (hsa_circ_0000005, hsa_circ_0007541, hsa_circ_0008199 and hsa_circ_0077536) were genuinely present (expressed) in ESCC patients and cell lines (see image 3 ). in image 3 A is the sequencing data of 4-circular RNA markers (hsa_circ_0000005, hsa_circ_0007541, hsa_circ_0008199, hsa_circ_0077536) in ESCC patient cancer tissue; image 3 B is the expression of 4-circular RNA markers (hsa_circ_0000005, hsa_circ_0007541, hsa_circ_0008199, hsa_circ_0077536) in cancer tissues and cell lines KYSE140, KYSE150, KYSE180, KYSE270 and KYSE410 of 8 randomly selected ESCC patients Cancer8-Cancer15. This provides a theoretical and experimental basis for the four circular RNAs as prognostic indicators for ESCC patients.

Embodiment 3

[0049] Example 3: Determination of the ability of 4-circular RNA markers to predict the prognosis of ESCC patients

[0050] In the sequencing data set, according to the expression levels of the four circular RNAs in ESCC patient cancer tissues, the risk score of ESCC patients was calculated, and ESCC patients were divided into high-risk group and low-risk group according to the median risk score, Kaplan-Meier Analysis of survival curve results showed that the overall survival of ESCC patients in the high-risk group was significantly lower than that in the low-risk group (1.91 years vs. 3.72 years, p Figure 4 A).

[0051] In the PCR verification data set, according to the expression levels of the four circular RNAs in ESCC patient cancer tissues, the risk score of ESCC patients was calculated, and ESCC patients were divided into high-risk resistance and low-risk resistance according to the median risk score, Kaplan- The results of Meier survival curve analysis also indicated th...

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Abstract

The invention relates to the technical field of biological medicine, and provides a molecular marker for prognosis prediction of esophageal squamous cell carcinoma and application thereof. An RNA-seq data set is used for screening out four circular RNA gene combinations with the maximum AUC value, and the four circular RNA gene combinations are named as 4-circular RNA markers. The study shows that the 4-circular RNA markers can independently predict the survival of ESCC patients; the accuracy of the traditional TNM staging evaluation on the ESCC patient prognosis is obviously improved; and the 4-circular RNA markers can be used as reference judging indexes of the esophageal squamous cell carcinoma prognosis survival time and prognosis survival risk prediction reference. Through studying the relationship between the 4-circular RNA markers and the clinical prognosis of the esophageal squamous cell carcinoma patients, a novel marker is provided for the esophageal squamous cell carcinoma prognosis judgment, so that the timely intervention treatment on the esophageal squamous cell carcinoma patients can be improved; the clinical prognosis of the patients is improved; and the important significance is realized in the clinical practice.

Description

technical field [0001] The invention relates to the technical field of biomedicine, and is a circular RNA marker for human esophageal squamous cell carcinoma, in particular to a circular RNA marker combination and its use in predicting the prognosis of esophageal squamous cell carcinoma. Background technique [0002] Esophageal cancer is one of the most common digestive tract malignancies, and its morbidity and mortality rank seventh and sixth, respectively, worldwide. Esophageal cancer is mainly divided into two main pathological types: esophageal squamous cell carcinoma (ESCC) and esophageal adenocarcinoma (EAC). The incidence of esophageal cancer has obvious regional and racial differences. ESCC It is the most common type of esophageal cancer in China, accounting for about 90%. In terms of mortality, the number of deaths from esophageal cancer in China accounts for half of the global esophageal cancer deaths. Therefore, ESCC is a major public health challenge facing the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12Q1/6851C12N15/113C12N15/11
CPCC12Q1/6886C12Q1/6851C12Q2600/158C12Q2600/118C12Q2600/178C12Q2531/113C12Q2565/125C12Q2521/107C12Q2563/107
Inventor 王伟伟姜国忠朱迪赵志华孙淼淼李军扩李文才张建营
Owner THE FIRST AFFILIATED HOSPITAL OF ZHENGZHOU UNIV
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