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Application of gene PeNAC67 in breeding of phalaenopsis aphrodite

A phalaenopsis and gene technology, which is applied to the application field of gene PeNAC67 in the breeding of Xiaolanyu phalaenopsis, can solve the problems of time-consuming and labor-intensive breeding methods, cannot efficiently obtain flower phenotypes, etc., achieves high efficiency, fast cost, and improves economic benefits. , the effect of broad application prospects

Active Publication Date: 2021-04-09
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] With the improvement of living standards, people pay more and more attention to plants and flowers, and different preferences for Phalaenopsis flower organ phenotypes (such as lip traits or petal traits) also endow it with significant economic benefits; however, traditional breeding methods Time-consuming and labor-intensive, it is impossible to efficiently obtain flower phenotypes that meet market demand. Therefore, those skilled in the art are committed to providing a gene that regulates the development of orchid flower organ flower type and its application in breeding

Method used

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  • Application of gene PeNAC67 in breeding of phalaenopsis aphrodite
  • Application of gene PeNAC67 in breeding of phalaenopsis aphrodite
  • Application of gene PeNAC67 in breeding of phalaenopsis aphrodite

Examples

Experimental program
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Effect test

Embodiment 1

[0035]Example 1 Cloning of a small Lanyu Phalaenopsis Penac67 gene

[0036]The total RNA is extracted with the total RNA in the wild-type Lanyu Phalaenopsis Pan Pan RNA using a commercially available reinforcing kit, and the total RNA is reversed into cDNA using the commercially available retrore reservoir. With the reverse transcribe of cDNA as a template, the strip of the gene encoding region 981 bp is obtained using the primer sequence shown in SEQ ID NO.4 and SEQ ID NO.5, and the PCR clon is obtained. As shown in SEQ ID NO.2, the nucleotide sequence encoded, as shown in SEQ ID NO.3, from 321 amino acid residues, a molecular weight of 37.1 kharton. The gene PENAC67 is 1352 bp, and the full length nucleotide sequence list is shown in SEQ ID NO.1, the sequence of the gene coding region such as SEQ ID NO. 2.

Embodiment 2

[0037]Example 2 Biological Information Analysis of Smalllan Phalaenopsis Penac67 Protein

[0038](1) Put the PENAC67 protein sequence into the NCBI database and retrieve homologous proteins.

[0039](2) Use https: / / www.genome.jp / tools-bin / clustalw to analyze the conservative domain between PENAC67 and homologous amino acid sequences, and the results show that the PENAC67 transcription factor has a typical NAM domain.

[0040](3) Select the amino acid sequence of different species, using Mega5, analyze the developmental relationship between PENAC67 system. As a result, PENAC67 and Lanke plant iron dendrobium, Shenzhen Praza in the same branch, home belly branch.

[0041](4) Use https: / / swissmodel.expasy.org / interactive to predict PENAC67 protein three-level structure, such asfigure 2 As shown, the PENAC67 protein tertiary structure predicts its role in which homologous dimer is formed.

Embodiment 3

[0042]Example 3 Wild-type Lanyu Phalaenopsis Penac67 Gene Organ Expression Mode Analysis

[0043]Extract the total RNA in the wild-type small Lanyu Phalaenoplahnorn, flower branches, leaves, sepals, petals, lip, and Jisan columns, using the reverse transcription kit to cDNA, using primer SEQ ID NO .6 and SEQ ID NO.7, Real-Time PCR detection, such asimage 3 Indicated. The results show that the expression of the gene is significantly higher than the nutrition organ.

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Abstract

The invention discloses an application of a gene PeNAC67 in breeding of phalaenopsis aphrodite. An encoded protein of the gene PeNAC67 has an NAM structural domain, and a protein sequence encoded by the gene PeNAC67 is as shown in SEQ ID NO.3.

Description

Technical field[0001]The present invention relates to the field of biological breeding, and in particular, the application of gene PENAC67 in Phalaenopsis Breeding in Xiaolan Yanyu.Background technique[0002]Phalaenopsis is a tree-lanarian, the Van Lankan, is originally produced in the tropical rainforest. It is a harem, the meat is developed, the stem is short, the leaf is thick, flat, color gorgeous, pattern Strange, the flower period can reach 90-150 days, is a treasure in tropical orchids, and the beauty of "Lanzhong Queen" is called. It is a widely cultivated planting as one of the world's important ornamental flowers. Phalaenopsis Equestris, also known as Phalaenopsis, Glorous orchid, Phalaenopsis, originating in the Philippines, because of its flowers, is Phalaenopsis breeding Excellent parents. Its dyeing group is consistent with most of the phalaces, for diploid (2n = 2x = 38), is one of the model species of Lanke flower morphological development mechanism, and is the first ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C12N15/11C07K14/415A01H5/02A01H6/62
CPCC07K14/415C12N15/8218C12N15/827
Inventor 明凤盖若男徐庆于娄玉霞
Owner SHANGHAI NORMAL UNIVERSITY
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