Three-degree-of-freedom pipetting device of closed cartridge and closed cartridge

Abstract

The present invention provides a three-degree-of-freedom pipetting device of a closed cartridge and a closed cartridge, which expands the degree of freedom of the pipetting device, so that more reagent holes can be used in the limited volume of the closed cartridge . The closed cartridge includes a reagent disc, and a plurality of reagent holes are arranged on the reagent disc; the pipetting device includes a pipetting head, a rotating platform, a first driving assembly and a second driving assembly; the rotating platform includes a rotating chassis, and a chassis bearing part, The rotating chassis is rotatably arranged on the supporting part of the chassis, and the rotating chassis is provided with a chute, and the pipetting head is slidably arranged in the chute; the first drive assembly is used to drive the pipetting head to slide along the chute and drive the rotating chassis Rotate relative to the chassis bearing part to drive the pipette head to rotate, so that the pipette head can be aligned with any reagent hole on the reagent plate; the second drive assembly is used to drive the rotating platform to move up and down and drive the pipette head to move up and down, so that the pipette head can be aligned Liquid aspiration or liquid injection is performed on the aligned reagent wells.

Description

technical field [0001] The invention relates to the technical field of gene sequencing, in particular to a three-degree-of-freedom pipetting device of a closed cartridge and a closed cartridge. Background technique [0002] Nucleic acid is the main carrier of life genetic information. DNA and RNA sequences are called genetic codes, which are the basis for analyzing gene structure, function and their interrelationships, and are also the most accurate basis for molecular diagnosis of clinical diseases. Gene sequencing is a new type of gene detection technology, which is also of great significance and value to clinical medicine. The mechanism research and clinical diagnosis of many diseases gradually rely on gene sequencing technology. Before gene sequencing, the sequencing samples need to be prepared. Initially, preparation was mostly done manually, but as the number of samples increased, so did the need for automation. [0003] There are mainly two types of existing automa...

AI Technical Summary

Problems solved by technology

[0004] In related closed cartridges, a two-degree-of-freedom pipetting device is usually used, wherein one degree of freedom is realized by the up-and-down movement of the pipette, and the other degree of freedom is realized by the rotation of the reagent disc. As a result, only one ring of reagent wells can be effectively used in the closed cartridge with limited volume, which greatly limits the number of reagent wells and leads to low preparation efficiency.

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