A kind of composition for inhibiting Aeromonas and its preparation method and application

A technology of Aeromonas and Monas, which is applied in the field of aquatic products and can solve the problems of endless emergence of drug-resistant strains

Active Publication Date: 2022-08-02
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the long-term use of antibiotics in production practice will lead to the emergence of drug-resistant strains in a short period of time

Method used

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  • A kind of composition for inhibiting Aeromonas and its preparation method and application
  • A kind of composition for inhibiting Aeromonas and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of Scutellaria chinensis extract

[0033] After smashing and sieving the Scutellaria japonica powder, accurately weigh 600g of the powder and heat it at 80°C for 20 minutes; then divide the heated powder into three parts of the same quality of Scutellaria japonica powder. The blood vine powder was added to 20 times the amount of ethanol with a concentration of 50%, and extracted for 12 hours to obtain an extract A. The second part of the vine root powder was added to 20 times the amount of ethanol with a concentration of 60%. After leaching for 12 hours, the extraction solution B was obtained, and the third part of the powder was added to 20 times the amount of ethanol with a concentration of 90%, and the extraction was performed for 48 hours to obtain the extraction solution C; The solution A, the extraction solution B and the extraction solution C are mixed evenly and then filtered, and the obtained filtrate is rotary-evaporated to obtain the ext...

Embodiment 2

[0043] Example 2 The antibacterial effect of the extract of Gallatia chinensis on Aeromonas hydrophila

[0044] 1. Preparation of culture medium

[0045] BHI medium was purchased from BD Company in the United States; 5% sheep blood agar plates, bacterial biochemical micro-identification tubes, etc. were purchased from Guangzhou Huankai Microorganism Co., Ltd.

[0046] 2. Preparation of bacterial suspension

[0047] Test bacteria culture: The Aeromonas hydrophila strain was activated, streaked on the solid medium with an inoculating needle, and after culturing at 30°C for 24 hours, a single colony was picked and inoculated on the nutrient broth medium for 24 hours with shaking. The bacterial solution was diluted to an appropriate concentration by the plate counting method, and the concentration of the bacterial solution was 1×108 CFU / mL.

[0048] 3. Drug susceptibility test

[0049]The drug susceptibility test adopts the agar diffusion method, and the bacterial suspension cu...

Embodiment 3

[0056] Embodiment 3 The influence of the ratio of the extract of Radix Glycyrrhizae and the extract of Qianjinpu on the antibacterial effect

[0057] After smashing and sieving the Scutellaria japonica powder, accurately weigh 600g of the powder and heat it at 80°C for 20 minutes; then divide the heated powder into three parts of the same quality of Scutellaria japonica powder. The blood vine powder was added to 20 times the amount of ethanol with a concentration of 50%, and extracted for 12 hours to obtain an extract A. The second part of the vine root powder was added to 20 times the amount of ethanol with a concentration of 60%. After leaching for 12 hours, the extraction solution B was obtained, and the third part of the powder was added to 20 times the amount of ethanol with a concentration of 90%, and the extraction was performed for 48 hours to obtain the extraction solution C; The solution A, the extraction solution B and the extraction solution C are mixed evenly and ...

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Abstract

The invention provides a composition for inhibiting Aeromonas, a preparation method and application thereof. Serve evenly. The composition for inhibiting Aeromonas according to the present invention contains doxycycline, Scutellaria chinensis extract and extract of Qianjinpu, and the combination of the three has obvious inhibition on Aeromonas bacteria The composition can be used for the preparation of medicaments and feed additives.

Description

technical field [0001] The invention belongs to the field of aquatic products, and in particular relates to a composition for inhibiting Aeromonas, a preparation method and application thereof. Background technique [0002] my country is a big country in aquaculture, and the aquaculture density and output of aquatic products are high. However, due to the backward aquaculture technology and facilities, it often leads to the frequent occurrence of bacterial diseases in fish. At present, antibiotic-based drug control is still the most effective and direct method in aquaculture production, and plays a very important role in the prevention and control of fish bacterial diseases. However, the long-term use of antibiotics in production practice will lead to the emergence of resistant strains in a short period of time. SUMMARY OF THE INVENTION [0003] In view of this, the present invention aims to overcome the defects in the prior art, and proposes a composition for inhibiting A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/486A61K9/14A23K10/30A23K20/195A23K50/80A61P31/04A61K31/65
CPCA61K31/65A61K36/486A61K36/48A61K9/14A61P31/04A23K10/30A23K20/195A23K50/80A61K2236/333A61K2300/00
Inventor 刘春李薇苏友禄江飚马杰秦真东黄燕华林蠡
Owner ZHONGKAI UNIV OF AGRI & ENG
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