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Alternaria alternata antagonistic actinomycete strain and application thereof

A technology of tobacco red spot disease and actinomycetes, which is applied in the direction of application, bacteria, and fungicides, can solve the problems of reducing the control effect and limiting the adverse impact of the ecological environment, and achieves the effects of high activity, stable inhibitory effect, and low cost

Active Publication Date: 2021-04-02
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the problems of resistance / resistance and pesticide residues of chemical agents, their decreasing control effects and adverse effects on the ecological environment limit long-term large-scale use; research and development of new high-efficiency biological pesticides for plant disease and insect pest control are green and sustainable crops. inevitable direction of development
The tobacco brown spot antagonistic actinomycete isolated from the tobacco rhizosphere soil in the present invention is Streptomyces flavusin F706, used as tobacco disease antagonistic bacteria to inhibit tobacco red spot, black shank, gray mold, bacterial wilt, The growth of wildfire disease and empty shank pathogenic bacteria has not been reported yet

Method used

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  • Alternaria alternata antagonistic actinomycete strain and application thereof
  • Alternaria alternata antagonistic actinomycete strain and application thereof
  • Alternaria alternata antagonistic actinomycete strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Strain Isolation

[0046] Tobacco rhizosphere soil was collected, sieved and ground, then air-dried (20°C, 3 weeks), weighed 5 g of naturally-air-dried soil sample, and mixed with CaCO 3 Mix evenly at a ratio of 10:1 (w / w), air-dry at 26°C for 7 days, and mix CaCO3 with 0.5 g of soil sample with 50 mL of phosphate buffer (10 mmol / L, pH 7.0, containing 10% soil juice) Make a soil suspension, shake at 30°C for 2 hours, then centrifuge at 1500 rpm for 20 minutes; after gradient dilution, take 10-5 0.15ml of the diluent was evenly spread on Gao’s No. 1 plate medium, and after 10 days of constant temperature cultivation at 28°C, single colonies of different shapes and colors were picked and preserved for future testing.

[0047] Bacteriostatic activity test

[0048] The important tobacco disease pathogens tested for antibacterial activity were isolated, identified and preserved in the fungal laboratory of Guizhou Tobacco Research Institute, including Ralstonia solanacearum,...

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Abstract

The invention discloses an alternaria alternata antagonistic actinomycete strain and an application thereof. The alternaria alternata antagonistic actinomycete strain is Streptomyces flavotricini F706, has a preservation name of the Streptomyces flavotricini F706, is preserved in the China Center for Type Culture Collection (CCTCC) at Luojia Mountain Wuhan University, Wuchang District, Wuhan City,Hubei Province on November 18, 2020, and has a preservation number of CCTCC No.M 2020755. A gene sequence of the alternaria alternata antagonistic actinomycete is a nucleotide sequence as shown in SEQ ID No.1.

Description

technical field [0001] The invention relates to the field of biological control of microorganisms and plant diseases, in particular to an antagonistic actinomycete strain against tobacco red spot disease and its application. Background technique [0002] Tobacco red spot disease is a tobacco leaf disease caused by fungal infection of the genus Alternaria spp. It occurs in the light of the epidemic range, has the characteristics of intermittent and explosive, and has a great impact on the yield and quality of tobacco leaves. It is an important issue in tobacco production. One of the most threatening diseases. Actinomycetes are a class of microbial resources with great economic value and wide application prospects. Because of their rich and diverse metabolites such as antibiotics, enzymes, and inhibitors, they have important application values ​​in the fields of agricultural and forestry production, industry, and clinical medicine. Mining biologically active actinomycete reso...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/28A01P3/00C12R1/465
CPCC12N1/20A01N63/28
Inventor 曹毅陆宁孟建玉杨志晓陈兴江商胜华孙光军
Owner GUIZHOU TOBACCO SCI RES INST
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