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Application of baicalein in preparation of medicine for treating malignant mesothelioma

A technology of malignant mesothelioma and baicalein, which is applied in the field of pharmacology, can solve the problems of malignant mesothelioma of baicalein that have not yet been seen, and achieve the effect of enhancing anti-tumor effect, enhancing sensitivity and reducing dosage

Inactive Publication Date: 2021-04-02
YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no relevant report on the use of baicalein in the treatment of malignant mesothelioma

Method used

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  • Application of baicalein in preparation of medicine for treating malignant mesothelioma
  • Application of baicalein in preparation of medicine for treating malignant mesothelioma
  • Application of baicalein in preparation of medicine for treating malignant mesothelioma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] In this example, the effects of baicalein on the migration and invasion of malignant mesothelioma cells were detected by in vitro wound healing assay and cell migration and invasion assay (Transwell).

[0029] Wound healing experiment in vitro: take the human malignant mesothelioma cell line MESO924 in the logarithmic growth phase, trypsinize and digest, resuspend in RPMI-1640 medium containing 10% fetal bovine serum (FBS), and inoculate in 6-well plates , placed in 5% CO 2 Cultured in a cell incubator. After the cells are close to confluence with each other, use the tip of a 200 μL pipette to make a straight scratch on the culture plate. Then add baicalein at different concentrations (5, 10, 25, 50 and 100 μM) for treatment, add DMSO as a control group, continue to culture for 48 hours, observe and take pictures under a microscope (Leica DMI 3000B).

[0030] Cell invasion test: In the invasion test, 45,000 (250 μL) MESO924 cells were inoculated into the Transwell coa...

Embodiment 2

[0033] In this example, the effect of baicalein on the proliferation ability of human malignant mesothelioma cells was detected by cell proliferation experiments.

[0034] The specific steps are as follows: resuspend MESO924 cells in RPMI-1640 medium containing 10% FBS, inoculate them in 96-well plates, add different concentrations (10, 25, 50 and 100 μM) of baicalein, add DMSO to the control group, and cultivate for 48 h Afterwards, detection was performed according to the operation manual attached to the cell proliferation kit (Progema).

[0035] The results showed that with the increase of baicalein concentration, the proliferation ability of MESO924 cells was significantly weakened ( figure 2 ), confirming that baicalein has obvious inhibitory effect on the proliferation ability of human malignant mesothelioma cells.

Embodiment 3

[0037] In this example, the effect of baicalein on the apoptosis of malignant mesothelioma cells was verified by flow cytometry detection.

[0038] In the apoptosis detection experiment, MESO924 cells treated with different concentrations (25, 50 and 100 μM) of baicalein for 48 hours were collected, washed with cold PBS, labeled with PI and Fluor 488annexin V for 15 minutes, and then used Alexa Fluor488annexin V / Dead Cell Apoptosis Kit (Thermo Fisher Scientifihc) was detected by flow cytometry, and analyzed by FlowJo software.

[0039] The results showed that after baicalein treatment, the apoptosis level of MESO924 cells was significantly increased, and the percentage of cell apoptosis in the 25 μM baicalein treatment group was 4.5%, and that in the 50 μM and 100 μM treatment groups reached 5.79 and 14.42%, respectively, while as a normal control Only 3.1% of cells in the DMSO treatment group were apoptotic ( image 3 ).

[0040] These test results confirmed that baicalein ...

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Abstract

The invention discloses application of baicalein in preparation of a medicine for treating malignant mesothelioma. According to the application, tests discover that the baicalein can be used for treating the malignant mesothelioma through inhibiting the migration, invasion and propagation of malignant mesothelioma cells and inducing apoptosis of the malignant mesothelioma cells, has obvious dose dependence and can be developed into the medicine for treating the malignant mesothelioma. According to the application, it is further discovered through the tests that the baicalein can be used for strengthening the sensitivity of the malignant mesothelioma cells to chemotherapeutics (cisplatin), so that the antitumor effect is strengthened, the dose of the chemotherapeutics is reduced, and toxicor side reactions are reduced.

Description

technical field [0001] The invention relates to the field of pharmacology, in particular to the application of baicalein in the preparation of medicines for treating malignant mesothelioma. Background technique [0002] Malignant mesothelioma is a highly aggressive malignant tumor of the pleura and peritoneum. At present, the treatment of malignant mesothelioma is mainly the comprehensive treatment of tumor debulking surgery or resection combined with radiotherapy and chemotherapy, but most of the patients are diagnosed at advanced stage, with a median survival time of 12 months and a poor prognosis. It is easy to relapse, the effect of radiotherapy is uncertain, and the side effects of chemotherapy drugs are large. Therefore, there is an urgent need for a new drug with low side effects that can effectively treat malignant mesothelioma. Traditional Chinese medicine, as an adjuvant or alternative treatment for malignant tumors, has a high degree of acceptance among malignant...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/352A61K33/243A61P35/00A61P35/04
CPCA61K31/352A61P35/00A61P35/04A61K33/243A61K2300/00
Inventor 李旭辉徐文飞柴颖杰王星华
Owner YANGTZE DELTA REGION INST OF TSINGHUA UNIV ZHEJIANG
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