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Hydrogen sulfide detection method based on colorimetric analysis and application

A detection method and colorimetric analysis technology, which is applied in the field of hydrogen sulfide detection based on colorimetric analysis, can solve problems such as difficulty in miniaturization, inability to achieve high-sensitivity detection of low-concentration targets, and long time consumption

Pending Publication Date: 2021-03-26
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] (1) Used to detect H 2 The existing methods of S, such as absorption spectroscopy, electrochemical method, gas chromatography, etc., are costly, time-consuming, require complex and expensive scientific instruments and professional operators, and are not easy to miniaturize;
[0010] (2) Currently, for H 2 There are few studies on immunoassays for S detection, and H 2 The detection of S in biological matrices is challenging, and high sensitivity detection of low-concentration targets cannot be achieved

Method used

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  • Hydrogen sulfide detection method based on colorimetric analysis and application
  • Hydrogen sulfide detection method based on colorimetric analysis and application
  • Hydrogen sulfide detection method based on colorimetric analysis and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Effects of NaAc-HAc buffers with different pH values ​​on the detection of hydrogen sulfide:

[0066] S101, Fe 3 o 4 The preparation steps are:

[0067] FeCl 2 4H 2 O (0.29815g, 50mmol / L), FeCl 3 ·6H 2 O (0.81087g, 100mmol / L) and deoxygenated HCl solution (1.2mmol / L, 30mL) were mixed, filled with nitrogen for 20min, and then quickly injected with deoxygenated NaOH solution (1.25mol / L, 30mL). 2 Stir vigorously for 2 hours under protection, and finally wash with secondary water three times until neutral.

[0068] The preparation steps of S102, MB-COOH are:

[0069] Add 1.0g of magnetic beads and 100mL of secondary water into a round bottom flask, and ultrasonically disperse; then, dissolve 2.5g of citric acid in the suspension, stir vigorously for 30min under nitrogen protection, and then heat to reflux at 70°C for 1h; Finally, magnetically separated and thoroughly washed with deionized water to obtain MB-COOH.

[0070] Activation of S103, MB-COOH and MB / PAMAM / Mn...

Embodiment 2

[0080] Hydrogen sulfide and MB / PAMAM / MnO 2 The effect of the reaction time of composite materials on the detection of hydrogen sulfide:

[0081] S101, Fe 3 o 4 The preparation steps are:

[0082] FeCl 2 4H 2 O (0.29815g, 50mmol / L), FeCl 3 ·6H 2 O (0.81087g, 100mmol / L) and deoxygenated HCl solution (1.2mmol / L, 30mL) were mixed, filled with nitrogen for 20min, and then quickly injected with deoxygenated NaOH solution (1.25mol / L, 30mL). 2 Stir vigorously for 2 hours under protection, and finally wash with secondary water three times until neutral.

[0083] The preparation steps of S102, MB-COOH are:

[0084] Add 1.0g of magnetic beads and 100mL of secondary water into a round bottom flask, and ultrasonically disperse; then, dissolve 2.5g of citric acid in the suspension, stir vigorously for 30min under nitrogen protection, and then heat to reflux at 70°C for 1h; Finally, magnetically separated and thoroughly washed with deionized water to obtain MB-COOH.

[0085] Activa...

Embodiment 3

[0095] A hydrogen sulfide detection method based on colorimetric analysis, comprising the following steps:

[0096] S101, Fe 3 o 4 The preparation steps are:

[0097] FeCl 2 4H 2 O (0.29815g, 50mmol / L), FeCl 3 ·6H 2 O (0.81087g, 100mmol / L) and deoxygenated HCl solution (1.2mmol / L, 30mL) were mixed, filled with nitrogen for 20min, and then quickly injected with deoxygenated NaOH solution (1.25mol / L, 30mL). 2 Stir vigorously for 2 hours under protection, and finally wash with secondary water three times until neutral.

[0098] The preparation steps of S102, MB-COOH are:

[0099] Add 1.0g of magnetic beads and 100mL of secondary water into a round bottom flask, and ultrasonically disperse; then, dissolve 2.5g of citric acid in the suspension, stir vigorously for 30min under nitrogen protection, and then heat to reflux at 70°C for 1h; Finally, magnetically separated and thoroughly washed with deionized water to obtain MB-COOH.

[0100] Activation of S103, MB-COOH and MB / P...

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Abstract

The invention discloses a hydrogen sulfide detection method based on colorimetric analysis and application. The method comprises the following steps: preparing ferroferric oxide; preparing magnetic bead-carboxyl; activating the magnetic bead-carboxyl to prepare an MB / PAMAM / MnO2 composite material; preparing a NaAcHAc buffer solution and TMB (tetramethylbenzidine); optimizing detection conditions;taking a sodium sulfide solution, adding a NaAc-HAc buffer solution, MB / PAMAM / MnO2 and TMB, carrying out colorimetric detection, and determining the absorbance. According to the hydrogen sulfide detection method based on colorimetric analysis and the application of the hydrogen sulfide detection method, the MB / PAMAM / MnO2 material has relatively strong catalytic performance, high sensitivity to H2Sdetection, strong universality, flexible operation, simple and convenient detection method and high economic benefit, and has important practical significance and development prospect in trace biological small molecule analysis; a simple, low-cost, rapid, high-sensitivity and stable-performance novel detection method is provided for clinical biomolecular detection.

Description

technical field [0001] The invention belongs to the technical field of colorimetric immunoassay, and in particular relates to a method for detecting hydrogen sulfide based on colorimetric analysis and its application. Background technique [0002] At present, the existing technologies commonly used in the industry are as follows: [0003] Hydrogen sulfide (H 2 S) is a colorless gas with the smell of rotten eggs, generally produced by incomplete combustion of sulfur-containing substances in industry, and is considered a toxic environmental pollutant. Recent studies have found that H 2 S is an important endogenous gas signal molecule in the living body and participates in many important physiological processes. H 2 Abnormal S concentration is associated with many diseases, such as Alzheimer's disease, Down syndrome, diabetes and liver cirrhosis. Traditional H 2 S detection methods include absorption spectroscopy, electrochemical methods, gas chromatography, and sulfur pre...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31G01N33/53
CPCG01N21/783G01N21/31G01N33/5308G01N2021/775
Inventor 刘冰倩苏丽霞杨雅妮罗大娟
Owner GUIZHOU UNIV
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