Porcine pseudorabies virus and porcine circovirus 3 double fluorescent quantitative PCR detection primer, probe, kit and method

Abstract

The invention provides a porcine pseudorabies virus and porcine circovirus 3 double fluorescent quantitative PCR detection primer, a probe, an amplification reagent and a method, and belongs to the technical field of virus detection. The primer probe comprises primers PRV gD real-F1, PRV gD real-R2, PCV3-real-F2 and PCV3-real-R2, and the nucleotide sequences of the fluorescent probes PRV gD and PCV3, and the PRV gD real-F1, PRV gD real-R2, PRV gD, PCV3-real-F2, PCV3-real-R2 and PCV3 are sequentially shown as SEQ ID No.1-6. The primer probe, the amplification reagent and the method have excellent sensitivity, specificity, repeatability and stability on detection of the porcine pseudorabies virus and the porcine circovirus 3.

Description

Technical field[0001]The invention belongs to the field of viral detection, and more particularly to a pig pseudo rabies virus and a pig ring pathois-detection primers and probes, kits and methods.Background technique[0002]Pig fake dog viruses seriously endanger pig industry, fast and accurately identify the diagnosis of the disease, which helps to prevent and control the diseases of animal diseases, the healthy development of animal husbandry is highly significant. Porcine circle virus 3 is a newly discovered pig pathogen in recent years, which can cause mother pig breeding disorders and caused pigs with only anorexia, multiple pimples and superficial phlegm.[0003]At present, although the related PCR and fluorescent quantitative PCR detection methods are established for the Pig Bogi Virus and Porcine Ring Virus 3, there is no Pig Pseudonovirus and a pig-free dual fluorescent quantitative PCR based on Taqman probes. Detection method. And the current piglet virus and pig-circular vir...

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Problems solved by technology

[0004] In view of this, the object of the present invention is to provide a kind of double fluorescent quantitative PCR detection primer and probe, kit and method of porcine pseudorabies virus and porcine circovirus type 3; Can detect porcine pseudorabies virus and porcine circovirus 3 simultaneously type, which effectively solves the shortcomings of the current double PCR detection method of porcine pseudodog virus and porcine circovirus type 3, such as low sensitivity and easy contamination, and the shortcomings of single-plex fluorescent quantitative PCR that can only detect one pathogen

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