A kind of selenium-resistant bacterial strain gx-d6 and its application

A technology of GX-D6 and strain, applied to selenium-resistant strain GX-D6 and its application field, can solve the problems of less research on microorganisms and the like

Active Publication Date: 2021-09-17
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Abstract
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Problems solved by technology

At present, domestic research on selenium mainly focuses on the behavior of selenium in soil, selenium-enriched plants and nano-selenium materials, but less research on microorganisms in selenium-enriched soils. For this reason, the inventors collected soil samples from selenium-enriched areas in Guangxi. Identify the selenium-resistant strains and measure the removal rate of selenium-resistant strains, in order to provide strain resources for the development of selenium resources from the perspective of microorganisms

Method used

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  • A kind of selenium-resistant bacterial strain gx-d6 and its application
  • A kind of selenium-resistant bacterial strain gx-d6 and its application
  • A kind of selenium-resistant bacterial strain gx-d6 and its application

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Embodiment

[0024] 1. Materials and methods

[0025] 1.1 Materials

[0026] 1.1.1 Bacteria screening soil

[0027] The bacteria screening soil was collected from four rice soil samples with different selenium content in Qintang District, Guigang City, Guangxi, and the four soils were named A, B, C, and D respectively. The soil to be tested was collected by the five-point mixing method to collect 0-20cm surface soil. After removing surface impurities, put it in a sterile ziplock bag and store it in an ice box. On the same day, it was brought back to the laboratory and stored in a 4°C refrigerator for later use. The physical and chemical properties of the soil are shown in the table 1.

[0028] Table 1 Soil physical and chemical properties and selenium content

[0029]

[0030] 1.1.2 Medium

[0031] Bacterial liquid medium: peptone 10g, beef extract 3g, sodium chloride 5g, distilled water 1L, pH 7.4-7.6.

[0032] Bacterial solid medium: peptone 10g, beef extract 3g, sodium chloride ...

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Abstract

The invention belongs to the technical field of microbiology, and in particular relates to a selenium-resistant bacterial strain GX-D6 and its application. A selenium-resistant bacterial strain GX‑D6, whose taxonomic name is Klebsiella pneumoniae subsp. rhinoscleromatis, was deposited in the Guangdong Microbial Culture Collection Center on December 10, 2020, with the preservation number GDMCC No.61356. The selenium-resistant bacterial strain Klebsiella pneumoniae subsp. rhinoscleromatis GX-D6 of the present invention can remove selenium in soil at a maximum of 40.04% at a selenium concentration of 4000 μg / mL. The results can provide bacterial strain resources for bioremediation of selenium polluted areas, and provide reference for the development and utilization of selenium resources.

Description

technical field [0001] The invention belongs to the technical field of microbiology, and relates to a selenium-resistant bacterial strain GX-D6 and an application thereof. Background technique [0002] Selenium is an essential trace element for the human body. Selenium deficiency will lead to an increase in the incidence of Keshan disease, cancer and cardiovascular diseases. Excessive selenium intake will cause hair loss and poisoning. Selenium mainly exists in four forms in nature, namely selenide (Se 2- ), elemental selenium (Se 0 ), selenite (SeO 3 2- ) and selenate (SeO 4 2- ), and the transformation of the valence state between various forms is closely related to microorganisms. The high-selenium environment is a potential source of selenium-tolerant microbial strains. Studies have shown that under high-selenium environmental stress, bacteria produce a stress response and convert highly toxic inorganic selenium into less toxic selenium [4] . Peng Zuoquan et al ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/22
CPCB09C1/10
Inventor 刘永贤黄婵婵潘丽萍邢颖廖青兰秀
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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