Preparation method of multi-layer coated probiotic microcapsule

A technology of microcapsules and probiotics, applied in the field of microorganisms, can solve the problems of porous surface, difficult to withstand high temperature and high pressure impact of feed pelleting, low survival rate of probiotics, etc., and achieve the effect of improving stress resistance

Active Publication Date: 2021-03-26
ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve the problem of low survival rate and instability of probiotics in use, the use of microcapsule embedding technology to protect probiotics has become a hot research topic at home and abroad.
However, most of the existing microcapsule coating technologies and products have the problem of surface porosity, especially the single-layer microcapsules have a pore size of 500-900 nanometers, and the stability is not good. After entering the human or animal digestive tract, most It is difficult to withstand the effects of gastric acid, bile acid and pancreatic juice with low pH value; when applied to livestock and poultry feed, it is difficult to withstand the high temperature and high pressure impact of feed pelleting; unable to meet requirements
In addition, some technical processes are not suitable for large-scale production, which seriously affects the activity of the coated probiotics and the industrialization and application of their products

Method used

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  • Preparation method of multi-layer coated probiotic microcapsule
  • Preparation method of multi-layer coated probiotic microcapsule
  • Preparation method of multi-layer coated probiotic microcapsule

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: the preparation of multi-layer coated Enterococcus faecium microcapsule product

[0027] (1) preparing concentration is the sodium alginate solution of 10g / L in the feeding tank of fermentation system;

[0028] (2) Weigh calcium carbonate at a ratio of 1:1 to the sodium alginate mass ratio, add it to the feeding tank, mix evenly, sterilize, then cool to 37°C, add Enterococcus faecium at the end of logarithmic growth Seed liquid, to a probiotic density of 1 x 10 6 -5×10 6 cfu / ml, fully mixed as the water phase;

[0029] (3) Add Span 80 as the oil phase to the liquid paraffin, the volume percentage of the Span 80 is 0.1%, and add the oil phase to the fermenter for sterilization;

[0030] (4) prepare the calcium chloride solution of 0.1mol / L in another feeding tank as a settling agent, and sterilize;

[0031] (5) At a stirring speed of 400rpm, according to the ratio of the volume ratio of the water phase to the oil phase of 1:3, slowly transfer the water ...

Embodiment 2

[0036] Embodiment 2: the preparation method of multi-layer coated Lactobacillus plantarum microcapsule product

[0037] (1) preparation concentration is the sodium alginate solution of 20g / L in the feeding tank of fermentation system;

[0038] (2) Weigh calcium carbonate at a ratio of 1:5 to the sodium alginate mass ratio, add it to the feeding tank, mix evenly, sterilize, then cool to 37°C, add Lactobacillus plantarum at the end of logarithmic growth Seed liquid, to a probiotic density of 1 x 10 6 -5×10 6 cfu / ml, fully mixed as the water phase;

[0039] (3) Add Span 80 as the oil phase to the liquid paraffin, the volume percentage of the Span 80 is 0.3%, and the oil phase is added to the fermenter, and sterilized;

[0040] (4) prepare the calcium chloride solution of 0.3mol / L in another feeding tank as a settling agent, and sterilize;

[0041] (5) At a stirring speed of 500rpm, according to the ratio of the volume ratio of the water phase to the oil phase of 1:5, slowly tra...

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Abstract

The invention discloses a preparation method of multi-layer coated probiotic microcapsules. The preparation method comprises the steps of coating probiotics before microencapsulation and coating afterfermentation, so as to prepare the multi-layer composite coated probiotic microcapsules. The preparation method disclosed by the invention plays a great role in maintaining the survival and stabilityof the probiotic preparation. In addition, the preparation method disclosed by the invention can more conveniently realize large-scale production of probiotic microencapsulated products, and is suitable for preparing probiotic microcapsules in multiple fields of feeds, foods, medicines and the like.

Description

technical field [0001] The invention belongs to the technical field of microorganisms. More specifically, it relates to a preparation method of multi-layer coated probiotic microcapsules. Background technique [0002] The function of live probiotic preparations mainly depends on the life activities of the live bacteria contained in them. Therefore, the content of live bacteria, the vitality of live bacteria and the length of shelf life are the key factors to evaluate the quality of products. At present, researchers at home and abroad regard maintaining the survival and stability of probiotic preparations as the standard for quality evaluation. In order to solve the problem of low survival rate and instability of probiotics in use, the use of microcapsule embedding technology to protect probiotics has become a hot research topic at home and abroad. However, most of the existing microcapsule coating technologies and products have the problem of surface porosity, especially t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/135A23K10/18A23K40/30A23P10/30A23P20/18A61K9/50A61K35/744A61K35/747A61K47/46A61P1/00
CPCA23L33/135A23K10/18A23K40/30A23P10/30A23P20/18A61K35/744A61K35/747A61K9/5026A61K47/46A61P1/00A23V2002/00A23V2400/157A23V2400/125A23V2400/173A23V2400/169A23V2200/06A23V2200/224A23V2200/3204A23V2250/022A23V2250/1578A23V2250/5026A23V2250/511A23V2250/5118A23V2250/5086A23V2250/51084A23V2250/51088A23V2250/636A23V2250/6406A23V2300/10
Inventor 李爱科王薇薇陈丽仙乔琳宋丹王丽
Owner ACAD OF NAT FOOD & STRATEGIC RESERVES ADMINISTRATION
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