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Application of an antioxidant in in vitro maturation of animal oocytes

An oocyte, in vitro mature technology, applied in the field of embryo engineering, can solve the problems of inability to obtain embryo sources, uneven egg quality levels, and low oocyte development ability.

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  • Abstract
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  • Application Information

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Problems solved by technology

[0003] However, oocytes obtained from either hormonal superovulation or abattoirs were significantly less developed than oocytes matured in vivo
The quality of these eggs is uneven, resulting in the inability to obtain a more stable source of embryos, which is the main defect of the current technology

Method used

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  • Application of an antioxidant in in vitro maturation of animal oocytes
  • Application of an antioxidant in in vitro maturation of animal oocytes
  • Application of an antioxidant in in vitro maturation of animal oocytes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Collection of adult sheep oocytes

[0023] After the ovaries were cut at the slaughterhouse, they were stored in saline at 37°C and shipped back to the experiment within two hours. The oocyte / cumulus cell complexes (COCs) in the ovary were aspirated with a 20G needle disposable syringe containing 2 mL of oocyte aspirating solution.

[0024] (2) Collection of lamb oocytes

[0025] Inject FSH (Ningbo Sansheng) six times for 4-8 week-old female lambs for 3 consecutive days, each injection of 45 mg, with an interval of 12 hours each time. The first injection of FSH was simultaneously injected with 330IU PMSG (Ningbo Sansheng). Surgical egg collection was performed 12 hours after the last FSH injection. Oocyte / cumulus cell complexes (COCs) in the ovary were aspirated in vivo with a 20G needle disposable syringe containing 2mL oocyte suction solution.

[0026] (3) In vitro maturation of oocytes

[0027] The selected COCs were washed three times in the oocyte suction ...

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Abstract

The invention discloses the application of an antioxidant in the maturation of animal oocytes in vitro. The method for improving the in vitro maturation of animal oocytes provided by the invention comprises: adding idebenone to the in vitro maturation liquid of animal oocytes. The invention also provides an application of idebenone in improving the in vitro maturation of animal oocytes. Specifically, the concentration of idebenone in the animal oocyte in vitro maturation solution is 10-30 μM; more specifically, it may be 20 μM. The method optimizes the in vitro culture condition of the oocyte and improves its embryonic development ability. The method is simple and easy, the material source is convenient, and it has important application value.

Description

technical field [0001] The invention belongs to the field of embryo engineering and relates to the application of an antioxidant in the in vitro maturation of animal oocytes. Background technique [0002] Animal embryo in vitro production technology is to collect mammalian oocytes from ovarian follicles, and carry out a series of technical processes such as in vitro maturation of oocytes in culture medium, in vitro fertilization, and in vitro culture of embryos. Now it has become increasingly mature and has become a Important animal reproduction biotechnology. This technology is generally used for pure multiplication and population expansion, increasing the number of breeding animals; shortening the breeding cycle of livestock, accelerating breed improvement; transplanting to produce twins, improving production efficiency; preserving excellent breed resources, etc. Therefore, this technology is a rapid breeding technology with great application value. [0003] However, ooc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/075
CPCC12N5/0609C12N2501/999C12N2501/31C12N2501/392
Inventor 侯健田浩刘克雄
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