Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Fengyuansu, composition including Fengyuansu, fusion gene encoding Fengyuansu, recombinant plasmid, recombinant bacterium and application

A technology of Fengyuansu and composition, which is applied in Fengyuansu, recombinant bacteria and its application fields, and can solve problems such as poor control effect

Active Publication Date: 2022-06-24
HEBEI AGRICULTURAL UNIV.
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the use of highly toxic pesticides for many years, pathogenic bacteria have developed resistance to pesticides, and the control effect has deteriorated.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Fengyuansu, composition including Fengyuansu, fusion gene encoding Fengyuansu, recombinant plasmid, recombinant bacterium and application
  • Fengyuansu, composition including Fengyuansu, fusion gene encoding Fengyuansu, recombinant plasmid, recombinant bacterium and application
  • Fengyuansu, composition including Fengyuansu, fusion gene encoding Fengyuansu, recombinant plasmid, recombinant bacterium and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Example 1 Extraction of lipopeptide compounds produced by Bacillus velesi HN-Q-8

[0060] A single colony was cultured at 37°C for 24h by the cross streaking method, and the newly activated HN-Q-8 strain was inoculated into 100mL LB liquid medium for 24h at 37°C and 200r / min to obtain seed liquid; the seed liquid was inoculated to In 100mL LB liquid medium, the inoculum amount is 10%, and continue to cultivate at 37°C, 200r / min for 72h: centrifuge the fermentation broth at 4°C, 8000rpm for 20min, collect the supernatant, and slowly add 6mol / min to the supernatant. The pH was adjusted to 2.0 with 1 L of HCl, and the precipitate was left to stand at 4°C overnight; then, the precipitate was collected by centrifugation at 8000 rpm at 4°C for 20 min, and the precipitate was dried in a fume hood and 30 mL of methanol was added to fully dissolve the precipitate. The methanol extract was filtered through a bacterial filter to obtain lipopeptide compounds.

Embodiment 2

[0061] Example 2 Bacteriostatic activity of lipopeptide compounds produced by Bacillus velesi HN-Q-8

[0062] The ZB4 strain of Potato nevus was used as the model bacteria, and the concentrations of lipopeptides were formulated into 10 mg / mL, 5 mg / mL, 1 mg / mL, 0.5 mg / mL, and 0.1 mg / mL. The model pathogenic bacteria cake (5 mm) was inserted in the center of the plate, and holes were drilled on both sides 2.5 cm away from the bacterial cake. Each concentration was a treatment, and each treatment was repeated 3 times, and an untreated blank plate was used as a control. When the blank control group is about to fill the petri dish, observe and take pictures.

[0063] The result is as figure 1 shown, figure 1 A is the bacteriostatic activity of lipopeptides at a concentration of 10 mg / mL; B is the bacteriostatic activity of 5 mg / mL lipopeptides; C is the bacteriostatic activity of 1 mg / mL lipopeptides; D is 0.5 mg / mL lipopeptides bacteriostatic activity of lipopeptide compounds;...

Embodiment 3

[0064] Example 3 Determination of antibacterial spectrum of lipopeptide compounds produced by Bacillus velesi HN-Q-8

[0065] 1. Determination of the inhibition of lipopeptide compounds on pathogens: the concentration of 10 mg / mL was used for the determination of the antibacterial spectrum. Fungal bacteria (5 mm) were inserted into the center of the plate, holes were drilled on both sides 2.5 cm away from the bacterial cake, and 50 μL of methanol was added to one side, and 50 μL of lipopeptide compounds were added to the other side. Each bacteria was treated as one treatment, and each treatment was repeated three times, and an untreated blank plate was used as a control. When the blank control group covered the petri dish, it was observed and photographed.

[0066] 2. Determination of the inhibition of lipopeptides against bacteria and actinomycetes: the dilution factor is 1×10 -5 of potato blackleg and the dilution factor is 1×10 -2 Take 100 μL of bacterial liquid from eac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a kind of Fengyuan element, fusion gene encoding lipopeptide compound, recombinant plasmid, recombinant bacteria and application, belonging to the technical field of plant disease prevention and control; ‑Q‑8, the preservation number of the Bacillus Velez HN‑Q‑8 is CGMCC No.19554. Bacillus Velez HN‑Q‑8 has high biocontrol effect. Among the antibacterial active substances produced by Bacillus Velez HN‑Q‑8, Fengyuan element plays the main antibacterial role. Fengyuansu has obvious inhibitory effect on potato black mole pathogen.

Description

technical field [0001] The invention belongs to the technical field of plant disease prevention and control, and particularly relates to fenggenin, a composition comprising fenggenin, a fusion gene encoding fenggenin, recombinant plasmids, recombinant bacteria and applications. Background technique [0002] Potato black scurf is caused by Rhizoctonia solani. In recent years, with the further increase of potato planting area in my country, the occurrence and damage of the disease have become increasingly serious in many major potato producing areas due to the inability to achieve crop rotation, which has seriously affected the appearance and commercial value of potato pieces. At present, prevention and control mainly rely on chemical agents. However, due to the use of highly toxic pesticides for many years, pathogenic bacteria have developed resistance to pesticides, and the control effect has deteriorated. Therefore, biological control has become the focus of current resea...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/32C12N15/32C12N15/62C12N15/70C12N1/21A01N37/46A01N63/20A01N63/60A01P3/00C12R1/19
CPCC07K14/32C12N15/70A01N37/46A01N63/20A01N63/60C07K2319/00A01N63/22
Inventor 朱杰华杨志辉赵冬梅杨毅清赵雅
Owner HEBEI AGRICULTURAL UNIV.
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com