Primer and probe sequence for fluorescence RAA detection of vibrio parahaemolyticus and application thereof

Abstract

The invention provides a primer and probe sequence for fluorescence RAA detection of vibrio parahaemolyticus, which is high in specificity, high in sensitivity and high in detection speed, and application thereof. A totally-closed reaction is performed, fluorescence data is monitored in real time, and subsequent treatment does not need to be carried out so as to avoid pollution and ensure the reliability of a detection result; and the primer and probe sequence is not depended on expensive instruments such as a PCR instrument and the like, even can detect at the normal temperature of 37 DEG C,can obtain a diagnosis result within 20 minutes, greatly shortens the detection time, can implement single-tube on-site and rapid detection, and has the advantages of high specificity, high sensitivity and high detection speed.

Description

Technical field [0001] The present invention belongs to the field of in vitro nucleic acid detection, and more particularly to primers and probes sequences for detection of velulent fluoris fluorescent RAA and their applications. Background technique [0002] Paragraph is a marine bacterium, mainly from fish, shrimp, crab, shellfish and seaweed and other sea products. Eat foods containing the bacteria can cause food poisoning, also known as thorningal bacteria. Clinically, with acute attack, abdominal pain, vomiting, diarrhea and water samples are main symptoms. Vibrio-solulent strain, Vibrio, Gram-dyed negative, coherent anaerobacteria, is a slightly curved arc. Pinophysique. The most suitable medium: temperature is 30 ° C to 37 ° C, salt containing 2.5% -3% (if the salt concentration is less than 0.5%, the pH is 8.0-8.5. The bacterium is sensitive to acids. When pH 6 is below, it is not growing, and L-3Min is death in ordinary vinegar. The colonies are often raised in solid med...

AI Technical Summary

Problems solved by technology

The reaction is carried out at 39°C for 20 minutes. In addition, RAA technology can also complete multiple primer amplification. With the fluorescence instrument, a set of RAA multiple fluorescence real-time detection system can be formed, that is, fluorescent labels of different colors can be used to detect in the same reaction. To different target genes, which is unmatched by other constant temperature nucleic acid amplification techniques or nested PCR techniques

At present, there is no disclosure of primers and probe sequences and kits for the detection of Vibrio parahaemolyticus fluorescent RAA at home and abroad.

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