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Wheat stripe rust resistance gene yrZ15-1949 as well as molecular marker and application thereof

A molecular marker and stripe rust technology, applied in the field of molecular biology, can solve the problems of loss of resistance of wheat varieties, the epidemic of stripe rust, and the threat to safe production of wheat, so as to increase the source of new stripe rust resistance, high utilization value, increase in Effects of wheat stripe rust resistance

Active Publication Date: 2021-03-02
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the frequent variation of stripe rust physiological races, the emergence of new toxic physiological races often leads to the rapid "loss" of resistance in wheat varieties, triggering the spread of stripe rust and threatening the safe production of wheat

Method used

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  • Wheat stripe rust resistance gene yrZ15-1949 as well as molecular marker and application thereof
  • Wheat stripe rust resistance gene yrZ15-1949 as well as molecular marker and application thereof
  • Wheat stripe rust resistance gene yrZ15-1949 as well as molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038]Example 1 Obtaining of the resistance gene yrZ15-1949 and its molecular marker KASP-Z1949-1

[0039]The present invention uses the cultivated one-grain wheat introgressive line Z15-1949 with the source of exogenous disease resistance genes as the female parent, and crosses the wheat variety ‘SY95-71’ as the male parent to obtain the hybrid F1, F1Obtain F by self-bred generation2, F2Self-bred to get F2:3Families, the population size is 124 lines, which constitute a genetic mapping population.

[0040]According to 55K SNP chip data, use JoinMap4.0 to construct a genetic map. Combining the population’s resistance to stripe rust phenotype data, using the Inclusive Composite IntervalMapping-ADD (ICIM-ADD) method in QTL IciMapping 4.0, setting the threshold LOD≥2.5, using the 2017 and 2018 two-year pairs F2, F2:3The phenotypic identification data of the family population of stripe rust was combined with wheat 55K SNP chip to construct a genetic map to locate the stripe rust resistance gen...

Embodiment 2

[0054]Example 2 The molecular marker KASP-Z15-1949-1 was used in the verification population ‘Z15-1949’ × wheat variety ‘AvocetS’ F2Application in

[0055]1) The F 2 verification population plant of an introgression line of wheat ‘Z15-1949’ × wheat variety ‘Avocet S’ was cultivated, and 80 individual plants were randomly selected from the progeny lines.

[0056]2) Perform KASP-Z15-19491 marker detection on the 80 individual strains obtained. The specific method is: extract the DNA of 80 strains; use it as a template to label the specific primers of KASP-Z15-1949-1 Perform PCR amplification on the primers and perform fluorescence readings, and the primers are:

[0057]Primer on FAM tag: (the underlined part is the FAM tag sequence) 5’--GAAGGTGACCAAGTTCATGCTTGGATGGTCGCTTTCCATTC-3’;

[0058]Primer on HEX tag: (The wavy line is the HEX tag sequence)

[0059]Universal downstream primer: 5'-AAGGAAAAGGAAAGACCAGA-3'.

[0060]The amplification system of the above PCR amplification is: 5μL Master Mix, three pr...

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Abstract

The invention discloses a wheat stripe rust resistance gene yrZ15-1949 as well as a molecular marker and application thereof. The wheat stripe rust resistance gene yrZ15-1949 is located on a wheat 7Dchromosome short arm, and the physical position of the gene yrZ15-1949 in a RefSeqv1.0 genome version is 43.66 Mb to 46.51 Mb. The SNP site of the molecular marker is located in a yrZ15-1949 interval,the polymorphism is A / G, and the molecular marker can accurately track the wheat stripe rust resistance gene yrZ15-1949. The invention further discloses a primer group for identifying the wheat stripe rust resistance gene yrZ15-1949, the primer group provided by the invention can be used for rapidly screening out a wheat variety or strain with the wheat stripe rust resistance gene yrZ15-1949 forbreeding, and the breeding process of a wheat disease-resistant variety can be greatly accelerated.

Description

Technical field[0001]The invention belongs to the technical field of molecular biology, and specifically relates to a wheat stripe rust resistance gene yrZ15-1949 and its molecular markers and applications.Background technique[0002]Wheat is an important food crop that feeds about one-third of the world's population. Wheat stripe rust is a worldwide important disease caused by wheat stripe rust fungus. There have been several pandemics of stripe rust in the history of our country, and severe years can cause wheat production to be reduced by more than 40%, or even no harvest. Due to the frequent variation of the physiological races of stripe rust, the emergence of new virulent races often leads to the rapid "loss" of resistance of wheat varieties, triggering a pandemic of stripe rust and threatening the safety of wheat production. The continuous and effective prevention and control of wheat stripe rust is a long-term international problem. Therefore, discovering and using new stripe r...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/11C12Q1/6895C12Q1/6858
CPCC07K14/415C12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 张连全刘登才张明虎柳欣黄林郝明甯順腙袁中伟姜博陈雪姣
Owner SICHUAN AGRI UNIV
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