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A kind of melon and fruit damping-off disease biocontrol bacteria bth05 and its bacterial agent, preparation method and application of bacterial agent

A bio-control bacteria and damping-off technology, applied in biochemical equipment and methods, microorganism-based methods, botanical equipment and methods, etc., can solve problems such as environmental pollution, and achieve simple preparation methods, good control effects, and field conditions. Ease of application

Active Publication Date: 2022-03-11
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the environmental pollution and human and animal safety problems brought about by the application of chemical pesticides, the present invention provides a kind of melon and fruit damping-off disease biocontrol bacteria BTh05 and its bacterial agent, the preparation method and application of the bacterial agent. It has obvious biological control effect on the damping-off disease of Solanaceae plants and Cucurbitaceae plants, especially it can effectively sterilize the seeds of pepper, tomato and cucumber, and prevent the propagation of pathogenic bacteria of melon and fruit damping-off disease, thereby saving a lot of cost and improving the production of melons and fruits. Maximize the benefits and have great application value for the cultivation of melons and vegetables

Method used

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  • A kind of melon and fruit damping-off disease biocontrol bacteria bth05 and its bacterial agent, preparation method and application of bacterial agent
  • A kind of melon and fruit damping-off disease biocontrol bacteria bth05 and its bacterial agent, preparation method and application of bacterial agent
  • A kind of melon and fruit damping-off disease biocontrol bacteria bth05 and its bacterial agent, preparation method and application of bacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1 Research on the Inhibition of Biocontrol Bacteria BTh05 to Pythium teratiflora and Pythium melon and fruit

[0024] Pythium teratiflora and Pythium melon fruit were cultured under the condition of PDA medium (potato sugar water agar medium), cultured upside down at 28° C. for 3 days, and the hyphae were grown for later use. Pick a single colony of biocontrol bacteria BTh05 and inoculate it into LB liquid medium to obtain a culture medium, and use LB liquid medium to adjust the concentration of the culture medium to 1.0×10 8 cfu / mL. Set up 6 groups of treatments: clean water treatment group, culture supernatant group, bacterial suspension (bacteria obtained by centrifugation of the culture medium were resuspended in an equal volume of LB liquid medium) group, 10-fold diluted culture medium group, and 50-fold Diluted culture medium group and 100-fold diluted culture medium group. Place fresh tomato leaves in each treatment group (5mL for each group), with the ...

Embodiment 2

[0028] Embodiment 2 A kind of bacterial agent containing biocontrol bacteria BTh05

[0029] A bacterial agent containing biocontrol bacteria BTh05, which has been preserved in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is It is CGMCC NO.20906, the storage date is October 19, 2020, it is classified as Bacillus thuringiensis, and the dosage form of the bacterial agent is a suspension concentrate;

[0030] The preparation method of bacterial agent comprises the steps:

[0031] (1) Inoculate the Bacillus thuringiensis BTh05 strain stored at -80°C into solid LB medium for activation, culture it upside down at 30°C±1°C overnight (or 24h), pick a single colony and inoculate it into liquid LB culture Shake culture at 30°C±1°C to the logarithmic phase to obtain seed liquid for fermentation, the concentra...

Embodiment 3

[0036] Seed soaking application of embodiment 3 bacterial agents in industrialized seedling cultivation

[0037] The test was divided into a biocontrol bacteria BTh05 treatment group and a control group. The test method was to mix 0.5 g of Pythium teratiflora and 0.5 g of Pythium melon and fruit cells with each liter of sterilized nutrient soil, and obtain the cultivation after fully mixing. Substrate, sow the seeds of the treatment group and the control group according to the conventional method and routinely manage the melon and fruit plants. Each group is treated with 33 plants, repeated 3 times, and the pepper damping-off disease caused by Pythium terstrogenita / Pythium melon and fruit was observed and counted after 14 days. Incidence and disease grade of tomato damping-off and cucumber damping-off. in,

[0038] Biocontrol bacteria BTh05 treatment group: the concentration was 1.0×10 10 The bacterial agent of Example 2 of cfu / mL was prepared into 100-fold dilution, 200-fol...

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Abstract

The invention relates to the technical field of microbial plant protection application, in particular to a biocontrol bacterium BTh05 for melon and fruit damping-off disease and its preparation method, and its application in the prevention and treatment of melon and fruit damping-off disease. The biocontrol bacterium BTh05 has been used in Deposited by CGMCC, the deposit number is CGMCC NO.20906, the deposit date is October 19, 2020, and the classification is named Bacillus thuringiensis. The preparation method of the bacterial agent includes obtaining seed liquid for fermentation, fermentation and adding additives . The biocontrol bacterium BTh05 and its agent of the present invention have obvious biological control effects on the damping-off disease of Solanaceae plants and Cucurbitaceae plants, especially can effectively sterilize the seeds of pepper, tomato and cucumber, and prevent and control the reproduction of pathogenic bacteria of melon and fruit damping-off disease , save a lot of cost, maximize the production benefits of melons and fruits, and have great application value for the cultivation of melons, fruits and vegetables.

Description

technical field [0001] The invention relates to the technical field of microbial plant protection application, in particular to a biocontrol bacterium BTh05 for melon and fruit damping-off disease and its agent, preparation method and application of the agent. Background technique [0002] The outbreak of damping-off has seriously affected the yield and quality of fruits and vegetables, and restricted the development of agricultural economy. At present, chemical pesticides and chemical fertilizers are the main means to prevent damping-off of melons and fruits and increase crop yields. However, many problems such as pesticide residues and environmental pollution caused by long-term use of chemical pesticides and chemical fertilizers make people very worried about food safety. As people pay more and more attention to pollution-free and pollution-free green food, biological control methods instead of chemical control methods have gradually been recognized by the general public....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/23A01P3/00A01C1/08A01G13/00C12R1/07
CPCC12N1/20A01N63/23A01C1/08A01G13/00
Inventor 谢雪迎张新建周方园周红姿张广志赵晓燕吴晓青范素素王加宁
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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