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Biocontrol bacterium BC04 as well as microbial inoculum, microbial inoculum preparation method and application thereof

A technology for biocontrol bacteria and inoculants is applied in the application field of microbial plant protection, and achieves the effects of simple preparation method, good control effect and convenient field application

Active Publication Date: 2021-02-09
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Aiming at the prevention and control of food safety hazards caused by the excessive application of chemical pesticides in plant diseases caused by the pathogen Pythium malatus, the present invention provides a biocontrol bacterium BC04 and its agent, preparation method and application of the agent. The fungicide has obvious biological control effect on corn stalk rot and / or solanaceous crop damping-off, especially can effectively disinfect the soil with Pythium terogenes pathogenic bacteria, and prevent corn stalk rot and / or solanaceous crops damping-off disease, thereby effectively reducing the use of chemical protective agents and saving a lot of cost, and has great application value for the cultivation of food crops and economic plants

Method used

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  • Biocontrol bacterium BC04 as well as microbial inoculum, microbial inoculum preparation method and application thereof
  • Biocontrol bacterium BC04 as well as microbial inoculum, microbial inoculum preparation method and application thereof
  • Biocontrol bacterium BC04 as well as microbial inoculum, microbial inoculum preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0023] Example 1 The research of biocontrol bacteria BC04 on the degradative effect of Pythium teratiflora

[0024] Cultivate the sporangia of Pythium malatus under the condition of CMA medium (corn agar medium), make it germinate, obtain zoospores, and quantify it to 2.0×10 5 cfu / mL, spare. Pick a single colony of biocontrol bacteria BC04 and inoculate it into LB liquid medium to obtain a culture medium, and use LB liquid medium to adjust the concentration of the culture medium to 2.0×10 8 cfu / mL. Set up 6 groups of treatments: clean water treatment group, culture supernatant group, bacterial suspension (bacteria obtained by centrifugation of the culture medium were resuspended in an equal volume of LB liquid medium) group, 10-fold diluted culture medium group, and 50-fold Diluted culture solution group and 100-fold diluted culture solution group were added to the quantitative spore liquid of Pythium terogenes in equal amounts to study its degradation effect on Pythium terg...

Embodiment 2

[0029] Embodiment 2 A kind of bacterial agent containing biocontrol bacteria BC04

[0030] A bacterium agent containing biocontrol bacteria BC04, said biocontrol bacteria BC04 has been preserved in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, The preservation number is CGMCC NO.20907, the preservation date is October 19, 2020, the classification is named Bacillus cereus, and the dosage form of the bacterial agent is wettable powder;

[0031] The preparation method of bacterial agent comprises the steps:

[0032] (1) Inoculate the BC04 strain of biocontrol bacteria stored at -80°C into LB solid medium, and culture it upside down at 30°C±1°C for 16 hours, pick a single colony, inoculate the single colony in LB liquid medium, and inoculate Cultivate at ±1°C for 18 hours to the logarithmic growth phase to obtain seed liquid for ...

Embodiment 3

[0038] Application of embodiment 3 bacterial agents in spraying soil

[0039] The test is divided into a biocontrol bacterium BC04 treatment group and a control group, and the test method is that every liter of nutrient soil (the nutrient soil is purchased from Piedman Company, the formula of the nutrient soil: organic matter content ≥ 45%, humic acid content ≥ 15%, PH value: 5.5 ~ 6.8, free water content ≤ 20%) add 10g / L bacterial agent (or 5g / L bacterial agent, 2g / L bacterial agent) and 1.0g Pythium malosterni mycelium (including zoospores) and mix , as the cultivation substrate after fully mixing; control group: add 1.0g Pythium teratiflora thalline (containing zoospores) to every liter of nutrient soil and mix it as the cultivation substrate, sow the above-mentioned treatment group and the control group seeds according to the conventional method and Routine management of corn, pepper and tomato, 30 plants in each group, repeated 3 times, observed and counted the incidence ...

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Abstract

The invention relates to the technical field of microbial plant protection application, in particular to a biocontrol bacterium BC04, a microbial inoculum thereof, a preparation method of the microbial inoculum and application of the microbial inoculum in prevention and treatment of crop diseases caused by pythium teratogenum, the biocontrol bacterium BC04 is preserved in China General Microbiological Culture Collection Center on October 19th, 2020, and the preservation number is CGMCC NO.20907, wherein the biocontrol bacterium BC04 is named as bacillus cereus in a classified manner; and the dosage form of the microbial inoculum is wettable powder. The preparation method comprises the following steps: preparing a seed solution, preparing a fermentation medium, performing fermention, preparing a powdery pre-preparation and preparing the microbial inoculum. By using the biocontrol bacterium BC04 or inoculant, the use of a chemical protective agent can be effectively reduced, a large amount of cost is saved, and the biocontrol bacterium BC04 or inoculant has important application value for cultivation of grain crops and economic plants.

Description

technical field [0001] The invention relates to the technical field of microbial plant protection application, in particular to a biocontrol bacterium BC04 and its agent, preparation method and application of the agent. Background technique [0002] In recent years, with people's increasing attention to food health and the continuous development of green and sustainable ecological agriculture, the demand for green food and green vegetables and fruits has greatly increased. [0003] Pythium terogenes is one of the main pathogens that cause corn stalk rot and Solanaceae damping-off, and it mainly harms the rhizomes of crop seedlings. Because of its wide range of hosts, high prevalence and high incidence, it is a devastating disease to the production of grain and vegetables. In addition, the humidity and temperature of cultivated land are too high, long-term continuous cropping, and varieties susceptible to diseases are all conducive to the reproduction and pathogenesis of thi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/085
CPCC12N1/20A01N63/22C12R2001/085C12N1/205
Inventor 谢雪迎张新建周方园周红姿张广志赵晓燕吴晓青范素素王加宁
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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