Automatic detecting and supplementing device for cell culture fluid

Abstract

The invention discloses an automatic detecting and supplementing device for a cell culture fluid. The device comprises a supporting table, a culture box, a buffer box and a liquid storage box are sequentially fixed to the upper surface of the supporting table from left to right, a submersible pump is arranged in the liquid storage box, the output end of the submersible pump is connected with a connecting pipe, a conversion mechanism fixed through a supporting frame is arranged between the culture box and the buffer box, the right surface of the conversion mechanism is connected with a water inlet pipe, the water inlet pipe is connected with the other end of the connecting pipe, the right surface of the conversion mechanism is connected with a water outlet pipe, the water outlet pipe is connected with the buffer box, shunting pipes are connected to the left side and right side of the buffer box, and the other end of the shunting pipe on the left side penetrates through the culture box and is fixedly connected with an adding mechanism through an L-shaped connecting pipe. With the device, the cell culture fluid can be added in real time, the uniformity degree of the cell culture fluidcan be kept, liquid in the cell culture box can be kept uniform, ventilation is provided for the cell culture box, and oxygen is continuously provided.

Description

technical field [0001] The invention relates to the technical field of culture fluid replenishment, in particular to an automatic detection and replenishment device for cell culture fluid. Background technique [0002] Cell culture refers to a method of simulating the internal environment (sterility, suitable temperature, pH and certain nutritional conditions, etc.) in vitro to enable it to survive, grow, reproduce and maintain its main structure and function. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. [0003] There are many types of cell culture, including aerobic bacterial cell culture. The existing cell culture needs to continuously add cell culture fluid to meet the nutrients needed for cell growth and reproduction. Now most of them are added manually, which is very inconvenient. Convenience, and the addition may be forgotten to affect the culture results. Contents of the invention [0004] (1) Pur...

AI Technical Summary

Problems solved by technology

[0003] There are many types of cell culture, including aerobic bacterial cell culture. The existing cell culture needs to continuously add cell culture fluid to meet the nutrients needed for cell growth and reproduction. Now most of them are added manually, which is very inconvenient. Convenience, and may be forgotten to add affect culture results

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