Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Anticoagulant active peptide derivative and preparation method and application thereof

A technology of anticoagulant activity and peptide derivatives, applied in the field of biomedicine, can solve problems such as short half-life, achieve long half-life, good protection of peptide activity, and high antioxidant performance

Active Publication Date: 2021-03-02
GUANGDONG OCEAN UNIVERSITY
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polypeptide factors with a molecular weight of less than 20kDa are easily filtered by the glomeruli during metabolism; when passing through the renal tubules, the polypeptide factors are partially degraded by proteases and excreted from the urine, so the half-life is short

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Anticoagulant active peptide derivative and preparation method and application thereof
  • Anticoagulant active peptide derivative and preparation method and application thereof
  • Anticoagulant active peptide derivative and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] 1. Synthetic raw materials and related reagents:

[0043] Fmoc-L-GLu(ODmab)-OH, Aimeijie Technology Co., Ltd.;

[0044] Fmoc-L-Asn(Trt)-OH, Suzhou Tianma Pharmaceutical Group;

[0045] Fmoc-L-Met(O 2 )-OH, Gill Biochemical (Shanghai) Co., Ltd.;

[0046] Fmoc-L-His(Boc)-OH, Gill Biochemical (Shanghai) Co., Ltd.;

[0047] Fmoc-L-Lys(Boc)-OH, Gill Biochemical (Shanghai) Co., Ltd.;

[0048] Fmoc-L-Phe-OH, Gill Biochemical (Shanghai) Co., Ltd.;

[0049]p-Hydroxycinnamic acid was purchased from Shanghai Jizhi Biochemical Technology Co., Ltd.

[0050] 2. The preparation of an anticoagulant active peptide derivative is synthesized by FMOC-short peptide solid-phase synthesis method, specifically:

[0051] S1: Weigh 0.8g AM Resin resin with a substitution degree of 0.4mmoL / g into a solid-phase synthesizer, add 10mL of anhydrous dichloromethane (hereinafter referred to as DCM), place on a shaker and shake for 30min to fully swell the resin ;

[0052] S2: Connect the first ...

Embodiment 2

[0062] A structural formula of an anticoagulant active peptide derivative is as follows:

[0063]

[0064] The preparation process of an anticoagulant active peptide derivative is the same as that in Example 1, and the purity of the obtained anticoagulant active peptide derivative is 96.4%.

Embodiment 3

[0066] A structural formula of an anticoagulant active peptide derivative is as follows:

[0067]

[0068] The preparation process of an anticoagulant active peptide derivative is the same as that in Example 1, and the purity of the obtained anticoagulant active peptide derivative is 96.9%.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an anticoagulant active peptide derivative and a preparation method and application thereof, and relates to the field of biological medicines. The amino acid sequence of the anticoagulant active peptide derivative is X-Phe-Lys-His-Met-Asn-GLu, wherein the terminal group X is p-hydroxycinnamic acid, 2-amino-3-(2, 3-dihydroxyphenyl) propionic acid and 2-amino-3-(4-hydroxy-3-methoxyphenyl) propionic acid. The anticoagulant active peptide derivative prepared by the method has high anticoagulant and thrombolytic activity, long half-life period and high stability, and has high antioxidant activity.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to an anticoagulant active peptide derivative and its preparation method and application. Background technique [0002] Thromboembolic disease is known as the invisible killer of cardiovascular and cerebrovascular diseases. It can affect various organs and systems of the whole body. About 3‰ people suffer from different forms of thrombotic diseases every year. The number of people who die of cardiovascular and cerebrovascular diseases in my country reaches 3 million every year. More than 51% of the total annual death toll worldwide is caused by it. 75% of the surviving patients are disabled, and more than 40% of them are severely disabled, which seriously threatens human health. [0003] Thrombus refers to a semi-clot-like substance formed on the surface of blood vessels or the intima of the heart during blood flow. Thrombotic diseases include arterial thrombosis, venous thro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/02C07K1/08C07K1/06C07K1/04A61K38/08A61P7/02A23L33/18
CPCA23V2002/00A61K38/00A23L33/18A61P7/02C07K7/02A23V2200/326
Inventor 苗艳丽李泳赵云涛胡章张兆霞廖铭能张刘
Owner GUANGDONG OCEAN UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com