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Full-automatic nucleic acid detection system

A detection system, fully automatic technology, applied in the direction of microbial determination/inspection, biochemical instruments, biochemical equipment and methods, etc., can solve the problems of low detection throughput, poor safety, low degree of automation, etc., to avoid cross-contamination, The effect of reducing manual operations

Pending Publication Date: 2021-02-26
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Therefore, the technical problem to be solved in the present invention is to overcome the defects of low automation, low detection throughput and poor security in the prior art, thereby providing a fully automatic nucleic acid detection system, including:

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Examples

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Embodiment 1

[0048] This embodiment provides a fully automatic nucleic acid detection system, such as figure 1 and figure 2As shown, it includes at least one consumable preparation unit 101, at least one sample pre-processing unit 102, at least one nucleic acid extraction unit 103, at least one system preparation unit 104, at least one nucleic acid detection unit 105, at least one sample post-processing unit 106 and multiple Delivery unit 107.

[0049] The consumables preparation unit 101 is connected to the sample preprocessing unit 102, the nucleic acid extraction unit 103 and the system preparation unit 104 respectively through the transfer unit 107, and is used to provide consumables to the sample pretreatment unit 102, the nucleic acid extraction unit 103 and the system preparation unit 104. Consumables preparation unit 101 includes but not limited to mobile orifice plate station (not shown), orifice plate stack (not shown), multi-axis manipulator (not shown), liquid dispensing devi...

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Abstract

The invention provides a full-automatic nucleic acid detection system. The full-automatic nucleic acid detection system comprises a sample pretreatment unit, at least one nucleic acid extraction unit,at least one system preparation unit, at least one nucleic acid detection unit, a consumable preparation unit, a sample post-treatment unit and a plurality of transfer units; the sample pretreatmentunit is used for transferring a to-be-detected sample to a nucleic acid extraction well plate; the nucleic acid extraction unit is used for extracting nucleic acid in the to-be-detected sample on thenucleic acid extraction well plate; the system preparation unit is used for mixing a nucleic acid amplification reagent with a nucleic acid from the nucleic acid extraction unit; the nucleic acid detection unit is used for carrying out a nucleic acid amplification and a detection on the nucleic acid from the system preparation unit; the consumable preparation unit is used for providing consumablesfor the sample pretreatment unit, the nucleic acid extraction unit and the system preparation unit; the sample post-treatment unit is used for receiving medical waste generated by the nucleic acid extraction unit and the nucleic acid detection unit; and a plurality of the transfer units are used for isolating or smoothly communicating the transfer units with other units and transferring materials. Due to the fact that all links of a nucleic acid detection are covered, harm to personnel is avoided and an efficiency is improved.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to an automatic nucleic acid detection system. Background technique [0002] In recent years, new and sudden infectious diseases caused by highly pathogenic pathogenic microorganisms (such as new coronavirus, Ebola virus, Crimean-Congo hemorrhagic fever virus, SARS, MERS, etc.) to human health and public health. [0003] Biosafety has been listed as an important part of many national security strategies, and building a biosafety prevention system is a major strategic demand of all countries. However, research on the microbiological characteristics, pathogenic mechanism, and molecular epidemiology of highly pathogenic pathogens is in its infancy, and scientific research reserves are insufficient. lacking. [0004] Research and development of ultra-high-throughput automated pathogen nucleic acid detection technology equipment that meets high-level biosafety requirements can not ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/34C12M1/12C12M1/00
CPCC12Q1/6806C12Q1/6851C12Q2563/143C12Q2523/308C12Q2563/149C12Q2531/113C12Q2563/107C12Q2561/113
Inventor 李金泽周连群李传宇张威姚佳张芷齐郭振李超
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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