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Application of staphylococcus nepalensis to degradation of residual sugar in fermentation industrial organic wastewater

A staphylococcus, fermentation wastewater technology, applied in water pollutants, biological water/sewage treatment, heating water/sewage treatment, etc. Effect

Active Publication Date: 2021-02-26
SHANDONG ZHUORAN BIOTECHNOLOGY CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Especially about the cultivation method of Staphylococcus Nepalis strains, and its ability to act on wastewater, especially high-salt fermentation wastewater, and to effectively treat wastewater, there is almost no literature disclosure
[0004] At present, regarding the treatment of high-salt fermentation wastewater, the following patent document CN109136095A discloses a waste water produced by fermentation of monosodium glutamate, using three kinds of microorganisms, which can effectively use the waste water to prepare microbial protein and purify the waste water at the same time, but whether these three kinds of microorganisms Whether it has high salt tolerance and has a good ability to purify wastewater in a high-salt environment, the above patents do not involve

Method used

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  • Application of staphylococcus nepalensis to degradation of residual sugar in fermentation industrial organic wastewater
  • Application of staphylococcus nepalensis to degradation of residual sugar in fermentation industrial organic wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] By adding high-salt-tolerant Staphylococcus nepalis to the high-salt fermentation wastewater treatment system, the purpose of rapidly degrading the residual sugar in the fermentation wastewater is achieved, and after the bacteria are removed by solid-liquid separation, the coarse salt contained in the fermentation wastewater is concentrated and recovered.

[0025] Get the glutamic acid wastewater containing 19.89% of salt (mass volume percentage, the same below), and the sugar content of 3.16% (mass volume percentage, the same below) into the fermenter, the initial pH is adjusted to 6.5 with ammonia water, and the fermentation temperature is 35°C , according to the amount of 1% (volume percentage, hereinafter the same) into Staphylococcus nepalese seed solution, after ventilating and culturing for 48 hours, its sugar content was reduced to 0.68% (mass volume percentage, hereinafter the same).

[0026] Adjust the pH of the fermentation broth to 3.5, heat it to 70°C, add 0...

Embodiment 2

[0028] The application of Staphylococcus nepalis in the rapid degradation of organic acid and / or amino acid fermentation to produce residual sugar in waste water under high-salt environment is as follows:

[0029] Take glutamic acid wastewater with a salt content of 14.91% and a sugar content of 2.75% and put it into a fermenter, adjust the initial pH to 5.0 with ammonia water, and ferment at a temperature of 33.5°C, insert Staphylococcus nepalese seed liquid at a ratio of 1%, and ventilate and cultivate After 33 hours, its sugar content dropped to 0.71%.

[0030] Adjust the pH of the fermentation broth to 3.8, heat it to 85°C, add 0.18‰ sodium polyacrylate, let it stand for 6 minutes, and separate the solid and liquid by plate and frame filter press, the dry weight of bacterial protein reaches 21.5g / L; Evaporation followed by fluidized bed drying at a drying temperature of 80°C to produce crude salt with an ammonium sulfate content of 9.53%.

Embodiment 3

[0032] The application of Staphylococcus nepalis in the rapid degradation of organic acid and / or amino acid fermentation to produce residual sugar in waste water under high-salt environment is as follows:

[0033] Take glutamic acid waste water with a salt content of 13.32% and a sugar content of 2.05% and put it into a fermenter, adjust the initial pH to 5.8 with ammonia water, and ferment at a temperature of 30°C, insert Staphylococcus nepalese seed liquid at a ratio of 1%, and ventilate and cultivate After 40 hours, its sugar content was reduced to 0.56%.

[0034] Through the solid-liquid separation of the disc centrifuge, after drying the bacteria, the dry weight reaches 28.7g / L, and the filtrate is firstly multi-effect evaporated, then dried in a fluidized bed at a drying temperature of 100°C, and the ammonium sulfate content is 92.06%. kosher salt.

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Abstract

The invention belongs to the technical field of microbial treatment of fermentation industrial organic wastewater, and particularly relates to application of staphylococcus nepalensis to degradation of residual sugar in the fermentation industrial organic wastewater. The staphylococcus nepalensis provided by the invention is named as (Staphylococcus nepalensis) A-A10-1, is preserved in China Center for Type Culture Collection on September 16, 2020, and the preservation number is CCTCC NO: M 2020505. The application of staphylococcus nepalensis to rapid degradation of residual sugar in wastewater in a high-salt environment, especially application to rapid degradation of residual sugar in organic acid and / or amino acid fermentation production wastewater in the high-salt environment is the key content to be protected by the invention. The strain disclosed by the invention has excellent high-salt resistance, is used for treating specific high-salt fermentation production wastewater, and has the remarkable advantages of low operation cost, low consumption, high efficiency, high adaptability, relatively stable operation, convenience in operation and management and the like.

Description

technical field [0001] The invention belongs to the technical field of microbial treatment of organic waste water in fermentation industry, and in particular relates to the application of staphylococcus nepalis in degrading residual sugar in organic waste water in fermentation industry. Background technique [0002] Regarding the application of Staphylococcus Nepalis, patent document CN105358141A has disclosed: a method for treating or preventing bacterial colonization or infection in a subject, the method comprising the following steps: injecting a therapeutically effective dose of prohexidine or its therapeutic An acceptable salt is administered to the subject, wherein the bacterial colonization or infection is caused by the bacterial agent. One of the bacterial agents is Staphylococcus nepalalis. [0003] There are few literature disclosures about other applications of Staphylococcus Nepalis. Especially about the cultivation method of Staphylococcus nepalis strain, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C02F101/38
CPCC02F3/34C02F2101/38C02F1/66C02F1/001C02F1/04Y02W10/10
Inventor 赵晨李昆仑赵林李宝君岳秋林苏乐
Owner SHANDONG ZHUORAN BIOTECHNOLOGY CO LTD
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