A strain of Lactobacillus longum sf-b-27 and its application
A technology of SF-B-27 and Lactobacillus bifidum, applied in the field of microorganisms, can solve the problems of increasing sleep time and high adhesion, and achieve the effects of increasing sleep time, high adhesion and relieving mental fatigue.
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Embodiment 1
[0039] Example 1 Isolation and identification of Bifidobacterium longum SF-B-27
[0040] 1. Bacteria source
[0041] Healthy baby stool.
[0042] 2. Isolation of strains
[0043] The fecal samples of healthy infants were diluted ten-fold, and 100 μL of each gradient was evenly spread on the MRS solid plate medium, then taken out and placed in an anaerobic tank, and cultured overnight at 37°C;
[0044] MRS solid plate medium includes the following components:
[0045] Peptone 10g, Beef Powder 5g, Yeast Powder 4g, K 2 HPO 4 ·7H 2 O2g, Triammonium Citrate 2g, Glucose 20g, CH 3 COONa 3H 2 O5g, MgSO 4 ·7H 2 O0.2g, MnSO 4 ·4H 2 0.05g, Tween 801mL, distilled water 1000mL;
[0046] The next day, there were colonies of different shapes and sizes on the plate. Pick a number of characteristic colonies of lactic acid bacteria for the second generation streaking purification treatment, and the plate was anaerobic activated for 1 day to obtain the culture, and then the strain id...
Embodiment 2
[0057] Embodiment 2 A kind of product for improving sleep rich in Bifidobacterium longum SF-B-27
[0058] A sleep-improving product rich in Bifidobacterium longum SF-B-27, the product contains Bifidobacterium longum SF-B-27, red date powder, astragalus powder, Codonopsis powder, gastrodia elata powder, isomalt oligosaccharide, among which long double The content of Fidobacteria SF-B-27 was 20 billion / g.
[0059] The preparation method of the product includes the following steps:
[0060] (1) Inoculate Bifidobacterium longum SF-B-27 into the MRS medium plate, the inoculation amount is 1%, after culturing at 37 ° C, centrifuge the MRS bacterial liquid, vacuum freeze-drying, and obtain bacterial powder;
[0061] (2) The auxiliary materials are compounded by red jujube powder, astragalus powder, Codonopsis powder, Gastrodia elata powder and isomalt oligosaccharide in a ratio of 1:1:1:1:1 to obtain a product.
[0062] (3) Mix the Bifidobacterium longum SF-B-27 bacteria powder pre...
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