Human pulmonary infection assessment method based on intestinal flora in excrement sample and application of human pulmonary infection assessment method

Abstract

The invention discloses a human pulmonary infection assessment method based on intestinal flora in an excrement sample. The method comprises the following steps: collecting the excrement sample of a tested person and extracting DNA; configuring a PCR reaction system and performing PCR amplification; establishing a standard curve; performing quantitative analysis to obtain the quantity of the intestinal flora in the excrement sample; and calculating the ratio of enterococcus to faecalibacterium prausnitzii, and assessing the illness degree and prognosis condition of the tested person by combining the ratio with the value of the bifidobacterium content (Ec / Fp-B). According to the faecalibacterium prausnitzii method, the ratio of the enterococcus to the faecalibacterium prausnitzii in combination with the bifidobacterium content (Ec / Fp-B) can be used for assessing the disease degree and prognosis condition of pulmonary infection patients. The method has the advantages that the ratio of enterococcus to faecalibacterium prausnitzii in the intestinal flora is combined with bifidobacteria to effectively assess and judge the disease degree and prognosis condition of the pulmonary infectionpatients, the method is noninvasive and non-interventional, the pain of the patients is relieved, the detection efficiency is high, the detection cost is low and the like.

Description

【Technical field】 [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for evaluating human lung infection based on intestinal flora in stool samples and its application in patients with new coronary pneumonia infection. 【Background technique】 [0002] Infection refers to the local tissue and systemic inflammatory response caused by bacteria, viruses, fungi, parasites and other pathogens invading the human body, including blood infection, central nervous system infection, respiratory tract infection, digestive tract infection, urinary tract infection, etc. [0003] Our study found that patients with lung infection, especially severe and critically ill patients, have intestinal microecological imbalance. The gut is the largest metabolic and immune organ in the body. More and more evidence shows that the gut can participate in the regulation of various diseases through the "gut-lung axis", "gut-brain axis" and "gut-liver axi...

AI Technical Summary

Problems solved by technology

At present, there is no set of methods to evaluate the degree of illness and prognosis of people with lung infection based on intestinal microbial indicators

Images