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Method for extracting high-purity mangiferin from mango leaves

A technology of mango leaf and mangiferin, which is applied in the field of extraction of functional plant components, can solve the problems of low solubility of mangiferin, reduced extraction rate, low extraction rate, etc., and is beneficial to large-scale industrial production, improved yield, and improved extraction rate. high rate effect

Active Publication Date: 2021-02-05
湖北博瑞生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method utilizes alkaline aqueous solution to extract mangiferin, which causes the solubility of mangiferin in the solvent to be low and reduces the extraction rate.
[0005] In summary, the current technical solutions for extracting mangiferin from mango leaves have problems such as poor safety or low extraction rate.

Method used

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  • Method for extracting high-purity mangiferin from mango leaves
  • Method for extracting high-purity mangiferin from mango leaves

Examples

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Effect test

Embodiment 1

[0048] A method for extracting high-purity mangiferin from mango leaves, comprising the following steps:

[0049] 1) Take the mango leaves with complete leaf shape and good elasticity, wash, drain, remove the petiole, dry at 40°C, pass through an 80-mesh sieve after crushing, and store in the dark for future use;

[0050] 2) Mix the treated mango leaves with 75% ethanol solution at a ratio of 1:5g / mL, heat to 40°C with microwaves, and perform ultrasonic extraction twice, with extraction power of 120w and extraction time of 15min;

[0051] 3) Combine the extracts, centrifuge the extracts at 4°C and 3500r / min for 15min, and take the supernatant;

[0052] 4) The supernatant was rotary evaporated at 45°C until the volume was 1 / 5 of the original extract to obtain a concentrated extract;

[0053] 5) AB-8 type macroporous adsorption resin was used to purify the concentrated extract, the loading volume was 12BV, and after the purification was completed, it was eluted with 75% ethanol...

Embodiment 2

[0057] A method for extracting high-purity mangiferin from mango leaves, comprising the following steps:

[0058] 1) Take the mango leaves with complete leaf shape and good elasticity, wash, drain, remove the petiole, dry at 40°C, pass through a 120-mesh sieve after crushing, and store in the dark for later use;

[0059] 2) Mix the treated mango leaves with 95% ethanol solution at a ratio of 1:15g / mL, heat to 60°C with microwave, and perform ultrasonic extraction 5 times respectively, with extraction power of 120w and extraction time of 45min;

[0060] 3) Combine the extracts, centrifuge the extracts at 0°C and 5000r / min for 45min, and take the supernatant;

[0061] 4) The supernatant was rotary evaporated at 60°C until the volume was 1 / 5 of the original extract to obtain a concentrated extract;

[0062] 5) AB-8 type macroporous adsorption resin was used to purify the concentrated extract, the loading volume was 9BV, and after the purification was completed, it was eluted wit...

Embodiment 3

[0066] A method for extracting high-purity mangiferin from mango leaves, comprising the following steps:

[0067] 1) Take mango leaves with complete leaf shape and good elasticity, wash, drain, remove the petiole, dry at 40°C, pass through a 100-mesh sieve after crushing, and store in the dark for later use;

[0068] 2) Mix the treated mango leaves with 80% ethanol solution at a ratio of 1:8g / mL, heat to 60°C with microwave, and perform ultrasonic extraction 5 times respectively, the extraction power is 250w, and the extraction time is 25min;

[0069] 3) Combine the extracts, centrifuge the extracts at 4°C and 4000r / min for 20min, and take the supernatant;

[0070] 4) The supernatant was rotary evaporated at 50°C until the volume was 1 / 5 of the original extract to obtain a concentrated extract;

[0071] 5) AB-8 type macroporous adsorption resin was used to purify the concentrated extract, the loading volume was 10BV, and after the purification was completed, it was eluted wit...

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Abstract

The invention discloses a method for extracting high-purity mangiferin from mango leaves. The extraction method comprises the steps of mango leaf drying and crushing, microwave heating, ultrasonic extraction, centrifugation, rotary evaporation, macroporous adsorption resin purification, concentration, freeze drying and the like, has the characteristics of simple operation, low cost, high extraction rate and the like, and has no special requirements on production equipment. The extraction solvent adopted by the invention has the characteristic of environmental protection, and has no adverse effect on the environment and human health. The mangiferin extracted by the method is high in purity, and has huge industrial value and good application prospect. According to the method, the mango leafresources can be fully utilized, a new thought is provided for comprehensive utilization of the mango leaf resources, the mangiferin product with high additional value can be produced, the income of orchard workers is increased, and the method has huge industrial value and good application prospects.

Description

technical field [0001] The invention relates to the field of extracting plant functional components, in particular to a method for extracting high-purity mangiferin from mango leaves. Background technique [0002] Mango leaves are the leaves of Anacardiaceae mango (Mangifera indica L.), mainly distributed in tropical and subtropical regions such as Guangdong, Guangxi, Yunnan, Hainan, Taiwan, etc., and have the advantages of wide distribution, low price, and easy availability of raw materials. Mango leaves contain ascorbic acid, tannins, mangiferin, flavonoids and other substances. Mango leaf extracts can inhibit the growth and reproduction of pyogenic coccus and Escherichia coli. Sores and other effects. [0003] Mangiferin, also known as Chinonin or Mangiferin, is a carboketone glycoside of tetrahydroxypyrrole, which belongs to diphenone flavonoids. Mangiferin is the main active ingredient in mango leaves. Studies have shown that mangiferin has significant anti-inflammat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D407/04
CPCC07D407/04
Inventor 刘家波李震宇朱华兴
Owner 湖北博瑞生物科技股份有限公司
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