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Method for rapidly obtaining pepper homozygous diploid

A diploid, pepper technology, applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of narrow application scope, long breeding method cycle, and difficult haploid breeding technology, and achieves less technical difficulty. , the effect of shortening the breeding process and avoiding the phenomenon of trait separation

Inactive Publication Date: 2021-02-02
CHENGDU ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a method for quickly obtaining homozygous diploid capsicum, which effectively solves the long cycle of traditional capsicum breeding methods, the difficulty of existing haploid breeding techniques, and the wide range of applications. narrow question

Method used

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  • Method for rapidly obtaining pepper homozygous diploid
  • Method for rapidly obtaining pepper homozygous diploid
  • Method for rapidly obtaining pepper homozygous diploid

Examples

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Effect test

Embodiment 1

[0047] A method for rapidly obtaining capsicum homozygous diploids, comprising the steps of:

[0048] (1) Choose Pepper F 1 Generation hybrid seeds: use Zhongjiao 107 (F 1 Hybrid varieties) seeds 100;

[0049] (2) Seed culture: add colchicine 600mg / L in MS medium, then sub-pack in a petri dish with a diameter of 9cm, about 1cm thick; 1 The hybrid seeds were sterilized with 70% alcohol for 40 seconds, dried and placed on the medium, 10 capsicum seeds were placed in each dish, and cultured in the dark at 25°C for 15 days until the seeds germinated and the cotyledons opened;

[0050](3) seedling cultivation: add colchicine 400mg / L in MS medium, then sub-package in the culture bottle of diameter 9cm, thick about 1cm; The seedling that step (2) obtains from the cultivation in step (2) Take it out from the base, wash it with sterile water, transfer it to a culture bottle, and continue to cultivate at 25°C for 13 hours under continuous light for 15 days until the first true leaf o...

Embodiment 2

[0067] A method for rapidly obtaining capsicum homozygous diploids, comprising the steps of:

[0068] (1) Choose Pepper F 1 Hybrid seeds of the first generation: use the pepper material 07-16 as the female parent, peel the buds for artificial emasculation, and cross pollen with the pollen of the male parent pepper material 07-24 to obtain F 1 Generation hybrid seeds, take F 1 Generation hybrid seeds 110 grains;

[0069] (2) Seed culture: Add colchicine 580mg / L in MS medium, then sub-package in a petri dish with a diameter of 9cm, about 1cm thick; 1 The hybrid seeds were sterilized with 75% alcohol for 45 seconds, dried and placed on the culture medium, about 10 capsicum seeds were placed in each dish, and cultured in the dark at 24°C for 10 days until the seeds germinated and the cotyledons opened;

[0070] (3) seedling cultivation: add colchicine 380mg / L in MS medium, then sub-package in the culture bottle of diameter 9cm, thick about 1cm; The seedling that step (2) obtain...

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Abstract

The invention provides a method for rapidly obtaining pepper homozygous diploid. The method comprises the following steps of (1) selecting pepper F1-generation hybrid seeds; (2) conducting seed culture; (3) conducting seedling culture; (4) conducting secondary culture of seedlings; (5) conducting plug transplanting; (6) detecting tetraploid; (7) planting in a greenhouse; and (8) conducting F2-generation screening. According to the method, the problems that a traditional pepper breeding method is long in period, an existing haploid breeding technology is large in difficulty, and the applicationrange is narrow are effectively solved.

Description

technical field [0001] The invention belongs to the technical field of vegetable breeding, and in particular relates to a method for rapidly obtaining homozygous diploid peppers. Background technique [0002] Capsicum is an annual or perennial herb of the Solanaceae Capsicum genus. It is native to Central and South America and was introduced to China in the 17th century. Its fruit is rich in carotene, vitamins and capsaicin, and has high nutritional value. Capsaicin is a secondary metabolite produced by peppers, which has the functions of dispelling cold, stopping dysentery, killing insects, enhancing appetite, and promoting digestion. Therefore, capsicum is regarded as an important vegetable and condiment, and is widely planted all over the world. my country's pepper planting area and annual output continue to increase, and it has become the largest pepper producer in the world, and the pepper industry has also become my country's largest vegetable industry. [0003] Wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 李丽平唐有万谭华强
Owner CHENGDU ACAD OF AGRI & FORESTRY SCI
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