Method for inducing lung injury models of experimental animals with different severity degrees by spraying modeling medicines with different dosages in trachea

An experimental animal, severity technology, applied in medical science, veterinary instruments, etc., can solve the problems of unstable lung injury state, obstacles to lung injury research, and high model mortality, and achieves good severity correspondence, stability and stability. Good consistency and avoid tedious operations

Inactive Publication Date: 2021-01-29
苏州西山中科药物研究开发有限公司 +1
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Problems solved by technology

The model established by this method has a high mortality rate, a low modeling rate, poor model consistency, and cannot stably and well simulate the clinical state of lung injury with different severity levels, which also brings new problems to the research on lung injury under different disease courses. to hinder

Method used

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  • Method for inducing lung injury models of experimental animals with different severity degrees by spraying modeling medicines with different dosages in trachea
  • Method for inducing lung injury models of experimental animals with different severity degrees by spraying modeling medicines with different dosages in trachea
  • Method for inducing lung injury models of experimental animals with different severity degrees by spraying modeling medicines with different dosages in trachea

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Experimental program
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Embodiment Construction

[0028] Divide the rats into 6 groups, 6 in each group (half male and half male), body weight 180-220g, respectively normal control group, BLM10mg / kg, 20mg / kg, 30mg / kg, 40mg / kg, 80mg / kg dose Group.

[0029] 1) The rats were induced anesthetized with isoflurane, and after the rats reached the anesthesia state, they were immediately placed supine on a sloping platform, so that the opening of the trachea could be found through the mouth of the rat;

[0030] 2) Use a cold light source to irradiate the rat's neck and jaw, so as to obtain sufficient light source for subsequent intraoral operations;

[0031] 3) Pull out the rat's tongue, press the tongue base with the tongue depressor, and find the opening of the trachea;

[0032] 4) Using an "intratracheal nebulizer", insert the needle-shaped head of the nebulizer directly into the rat's trachea 4cm, observe the breathing pattern of the rat, and quickly inject the above-mentioned different doses of Bleomycin at the moment the rat in...

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Abstract

The invention provides a method for inducing lung injury models of experimental animals with different severity degrees by spraying modeling medicines with different dosages in trachea. The method comprises the following steps that an experimental animal is anesthetized, and after the animal reaches an anesthetized state, the animal is immediately held on a slope holding table in a supine mode sothat a tracheal opening can be found smoothly through the oral cavity of the animal; the mandible of the neck of the animal is illuminated by using a cold light source (or adopting a laryngoscope) soas to obtain a sufficient light source during subsequent intra-oral operation; the tongue of the animal is pulled out, the tongue root is pressed by a tongue depressor, and the trachea opening is found; an intra-tracheal atomizer is used, a needle-shaped head of the atomizer is directly inserted into the trachea of an animal to a certain degree (the size of the animal determines the insertion depth), the respiratory law of the animal is observed, modeling drugs of different doses are rapidly sprayed into the trachea at the moment that the animal inhales, and then the animal is kept on the slope fixing table for 5-30 seconds. The experimental animal lung injury model which is stable, controllable in death rate, better in consistency and capable of simulating clinical lung injuries of different severity degrees is obtained, and a more reliable animal disease model is provided for medical pre-clinical research.

Description

technical field [0001] The invention relates to a modeling method of an animal disease model, in particular to a method for inducing a lung injury model of an experimental animal with different degrees of severity by intratracheal spraying of different doses of modeling drugs. Background technique [0002] A stable lung injury model with controllable severity and mortality and good consistency is an important carrier and basis for preclinical research on drugs for lung diseases. At present, the commonly used animal model of lung injury is the method of puncturing the trachea after the trachea is exposed by neck surgery, and then instilling the modeling drug into the trachea. The model established by this method has a high mortality rate, a low modeling rate, poor model consistency, and cannot stably and well simulate the clinical state of lung injury with different severity levels, which also brings new problems to the research on lung injury under different disease courses....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/04
CPCA61D7/04
Inventor 彭定国高岩嵩郑霞金俊杰陈嘉诚陈媛王琳白瑜珊
Owner 苏州西山中科药物研究开发有限公司
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