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A kind of complex bacterial agent fermentation product and its preparation method and application

A technology of fermented products and compound bacterial agents, applied in the directions of bacteria, applications, and lactobacilli used in food preparation, can solve the problems of microbial contamination, low nutritional activity content of feed, etc., achieve the improvement of peptide substances, reduce the probability of occurrence, The effect of process green environmental protection

Active Publication Date: 2021-07-13
四川特驱农牧科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In view of the above-mentioned shortcomings, the present invention solves the defect that the product is easily contaminated by microorganisms in the production process by adding antimicrobial peptides to the combined fermentation animal feed, and also solves the problem of high-cost high-temperature sterilization in the traditional feed processing process. The technical problem of the low nutritional activity content of the feed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A kind of complex bacterial agent fermentation product

[0031] (1) Antibacterial stage: Select 70kg of high-quality 46 soybean meal, mix it with 30kg of dry bran (medium bran), and pre-dilute with water to prepare a solid medium, then add 0.1kg of antimicrobial peptide, 10kg of glucose oxidase, 50kg of glucose and water Mix well, and then ferment at 32°C for 18 hours to obtain the fermented material for later use;

[0032] (2) Solid-state fermentation stage: Add 1 / 10 times the amount of compound strains and 1 / 60 times the amount of cellulase to the fermentation material obtained in the antibacterial stage. It is mixed at a ratio of 1:1:1; water is added to humidify again, sealed and stored, and fermented at a constant temperature of 32°C for 60 hours to obtain a composite bacterial agent fermentation product, and the water content of the composite bacterial agent fermentation product is 37.5%.

Embodiment 2

[0034] A kind of complex bacterial agent fermentation product

[0035] (1) Antibacterial stage: Select 60kg of high-quality 46 soybean meal, mix it with 40kg of dry bran (medium bran), and pre-dilute with water to prepare a solid medium, then insert 0.1kg of antimicrobial peptide, 10kg of glucose oxidase, 20kg of glucose and water Mix well, then ferment at 30°C for 24 hours to obtain the fermented material for later use;

[0036] (2) Solid-state fermentation stage: Add 1 / 10 times the amount of compound strains and 1 / 60 times the amount of cellulase to the fermentation material obtained in the antibacterial stage. It is mixed at a ratio of 1:1:1; water is added to humidify again, sealed and stored, and fermented at a constant temperature of 30°C for 72 hours to obtain a composite bacterial agent fermentation product, and the water content of the composite bacterial agent fermentation product is 35%.

Embodiment 3

[0038] A kind of complex bacterial agent fermentation product

[0039] (1) Antibacterial stage: Select 80kg of high-quality 46 soybean meal, mix it with 20kg of dry bran (medium bran), and pre-dilute with water to prepare a solid medium, then insert 0.1kg of antimicrobial peptide, 10kg of glucose oxidase, 100kg of glucose and water Mix well, and then ferment at 35°C for 12 hours to obtain the fermented material for later use;

[0040](2) Solid-state fermentation stage: Add 1 / 10 times the amount of compound strains and 1 / 60 times the amount of cellulase to the fermentation material obtained in the antibacterial stage. It is mixed at a ratio of 1:1:1; water is added to humidify again, sealed and stored, and fermented at a constant temperature of 30°C for 72 hours to obtain a composite bacterial agent fermentation product, and the water content of the composite bacterial agent fermentation product is 40%.

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Abstract

The invention discloses a compound bacterial agent fermentation product and its preparation method and application. The preparation method of the composite bacterial agent fermentation product includes an antibacterial stage and a solid-state fermentation stage. 46 Soybean meal and bran are mixed, pre-diluted with water to obtain a solid medium, and then antimicrobial peptides, glucose oxidase, glucose and water are fully mixed, fermented for 12-24 hours, and fermented materials are obtained for later use. Add Lactobacillus plantarum, Saccharomyces cerevisiae, Bacillus subtilis, and cellulase to the fermentation material, add water again to humidify it, seal it and store it, and leave it to ferment at a constant temperature of 35°C for 48h~72h to obtain the fermentation product of the complex bacterial agent. The fermentation product of the compound bacterial agent prepared by the method of the present invention has high biological activity, and the preparation of functional animal feed by mixing the fermentation product of the compound bacterial agent with puffed corn can not only improve the nutritional efficacy of the feed, but also reduce the incidence of animal diseases and effectively replace some The use of antibiotics has a simple and easy process and is environmentally friendly, so it has a wide application prospect in the feed industry and aquaculture industry.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and in particular relates to a fermentation product of a composite bacterial agent and a preparation method and application thereof. Background technique [0002] In animal husbandry production, the application of antibiotics can improve feed utilization efficiency and economic benefits. However, the abuse of antibiotics in feed causes drug resistance and double infection of pathogenic microorganisms in livestock and poultry; drug residues in livestock and poultry products seriously affect product quality and safety, threatening human health. Therefore, it is necessary to look for efficient and green alternatives to antibiotics It has become a research hotspot. Since the 1990s, many researchers at home and abroad have begun to look for alternatives to antibiotics. Coupled with the shortage of conventional raw material resources and limited utilization, it is an inevitable trend t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/14A23K10/18A23K10/30A23K10/37A23K20/147A23K20/163A23K20/189A23K40/10
CPCA23K10/12A23K10/14A23K10/18A23K10/37A23K20/189A23K20/163A23K20/147A23K10/30A23K40/10A23V2400/169Y02P60/87
Inventor 王玲玲梅绍锋袁敏王贵芬王成刘贵莲钟霞陈柳林玉聪
Owner 四川特驱农牧科技集团有限公司
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