Multi-protein combined biomarker, application and heart valvular disease complicated with atrial fibrillation diagnostic kit
A biomarker and atrial fibrillation technology, applied in the field of molecular biology, can solve time-consuming and labor-intensive problems
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Embodiment 1
[0173] 1. Collection of Human Plasma Samples
[0174] Patients with heart disease and atrial fibrillation were the experimental group, and patients who maintained sinus rhythm were the control group. Blood samples were collected from all patients (experimental group and control group) in the morning before the operation, under the condition of fasting>10h. 2ml of whole blood was collected from each patient's peripheral vein, and immediately centrifuged at 2500rpm for 10 minutes to separate the upper layer of plasma. The separated plasma was then divided into several equal parts, placed in plasma collection tubes, and stored in a -80°C refrigerator for testing.
[0175] 2. Proteomic detection
[0176] 1) Plasma proteome detection
[0177] Proteins in each mixed sample of the two groups were detected by iTRAQ combined with multidimensional liquid chromatography-mass spectrometry (LC-MS / MS) (Applied Biosystems, Foster City, CA).
[0178] 2) Sample preparation
[0179] a) Pur...
Embodiment 2
[0235] Enzyme-linked immunosorbent assay (ELISA)
[0236] Apolipoprotein A1, angiotensinogen, LIM domain protein 7, macrophage colony-stimulating factor receptor 1, vitronectin, and prothrombin fragment F1+2 were detected by ELISA in freshly collected plasma samples. verified.
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