Cartilage membrane autosecretion factor retention culture dish

Abstract

The invention discloses a cartilage membrane autosecretion factor retention culture dish which structurally comprises a culture body and a semi-permeable membrane body, wherein the culture body is connected with the semi-permeable membrane body in a clamped mode, a certain distance is reserved between the bottom of a membrane cavity in the bottom of the semi-permeable membrane body and the bottomof a square dish at the bottom of the culture body, and the connecting edge of the culture body and the semi-permeable membrane body is designed to be clamped through a stepped bayonet; the semi-permeable membrane body is composed of a supporting component and a semi-permeable membrane, the semi-permeable membrane is formed by wrapping the supporting component with a fiber filtering layer, and thewhole cavity gap is divided into an upper membrane space body and a lower membrane space body; and the space between the lower membrane space body and the bottom of the square dish is used as a cellplanting space body. Cells are planted in a cell planting space body, corresponding culture media and factors are added, then a semi-permeable membrane body is installed, water molecules and harmful molecules can slowly reach the upper membrane space body through permeation gradient, and the purpose of changing liquid is achieved by changing the liquid in the space body on the membrane every day;and meanwhile, the cartilage membrane autosecretion factor retention culture dish is reasonable and simple in structural design, easy to implement and suitable for application and popularization.

Description

technical field [0001] The invention relates to the technical field of a perichondrium sheet culture dish, in particular to a culture dish for retaining autocrine factors of a perichondrium sheet. Background technique [0002] Articular cartilage is mainly avascular tissue composed of chondrocytes and extracellular matrix. It mainly relies on joint movement and extrusion to absorb nutrients. Therefore, the self-repair ability of cartilage damage is relatively weak. Clinical research has limited patient tissue materials, and also because Chondrocytes are terminally differentiated cells with limited proliferation ability in vitro and are prone to dedifferentiation. Dedifferentiation refers to the process in which differentiated cells lose their unique structure and function and become undifferentiated cells. Therefore, improving and maintaining chondrocyte phenotype and promoting chondrocyte chondrification play a pivotal role in the clinical application of chondrocytes. [0...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to solve the above problems and provide a culture dish for retaining autocrine factors of the perichondrium sheet. Through a simple structural design, the problem of high cost in the chondrogenic induction process can be reduced, and the possibility of changing the liquid can be reduced. Pollution problems and heavy workload problems

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