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Method for culturing dedifferentiated or undifferentiated thyroid cancer organoids and thyroid cancer culture medium

A thyroid cancer and culture medium technology, applied in the field of biomedicine, can solve problems such as lack of iodine uptake ability, poor prognosis of thyroid cancer patients, and dedifferentiation of DTC

Active Publication Date: 2021-09-10
北京科途医学科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, after DTC metastasizes, some DTCs will dedifferentiate and develop into dedifferentiated thyroid cancer.
[0004] Dedifferentiated and undifferentiated thyroid cancer cells do not have the ability to uptake iodine, so 131 I radiotherapy non-response, resulting in poor prognosis of patients with dedifferentiated or anaplastic thyroid cancer, for example, anaplastic thyroid cancer has a 5-year survival rate of less than 10%, and a 12-month mortality rate of 80.7%

Method used

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  • Method for culturing dedifferentiated or undifferentiated thyroid cancer organoids and thyroid cancer culture medium
  • Method for culturing dedifferentiated or undifferentiated thyroid cancer organoids and thyroid cancer culture medium
  • Method for culturing dedifferentiated or undifferentiated thyroid cancer organoids and thyroid cancer culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] This example demonstrates the culture of undifferentiated thyroid carcinoma organoids.

[0049] (1) Preservation and transportation of undifferentiated thyroid cancer tissue samples

[0050] The puncture tissues of patients with clinically confirmed anaplastic thyroid carcinoma were obtained as anaplastic thyroid carcinoma tissue samples. Undifferentiated thyroid cancer tissue samples were stored in thyroid cancer medium and transported to the operating room within 48 hours of cold chain at 4 degrees.

[0051] (2) Obtaining undifferentiated thyroid cancer tissue cells

[0052] Place the cleaned undifferentiated thyroid carcinoma tissue sample in a sterile petri dish, add the enzymatic hydrolysis solution (DMEM / F12 containing 2 volume % FBS and 2 mg / ml collagen hydrolytic enzyme) after filtration sterilization and warm bath at 37 °C culture medium), and use sterile instruments to cut the undifferentiated thyroid cancer tissue sample into minced shape to obtain the enzy...

Embodiment 2

[0058] The undifferentiated thyroid cancer organoids were cultured according to the method of Example 1, except that in the thyroid cancer medium used in this example, the concentration of nicotinamide was 5 mM, and the concentration of BM-Cyclin was 5 μg / ml.

Embodiment 3

[0060] The undifferentiated thyroid cancer organoids were cultured according to the method of Example 1, except that in the thyroid cancer medium used in this example, the concentration of nicotinamide was 20 mM, and the concentration of BM-Cyclin was 30 μg / ml.

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Abstract

The present disclosure relates to a method for culturing dedifferentiated or undifferentiated thyroid cancer organoids, the method comprising mixing dedifferentiated or undifferentiated thyroid cancer tissue cells with thyroid cancer medium and Matrigel to obtain a culture to be cultured, wherein the The thyroid cancer medium contains nicotinamide and BM-Cyclin, based on the thyroid cancer medium, the concentration of the nicotinamide is 5-20 mM, and the concentration of the BM-Cyclin is 5-30 μg / ml; The cultures were culture-expanded to obtain dedifferentiated or undifferentiated thyroid cancer organoids. In the method for culturing dedifferentiated or undifferentiated thyroid cancer organoids provided by the present disclosure, since the thyroid cancer medium used contains specific concentrations of nicotinamide and BM-Cyclin, this is beneficial to promote dedifferentiated or undifferentiated thyroid cancer organoids Therefore, when using the method provided by the present disclosure to culture dedifferentiated or undifferentiated thyroid cancer organoids, the culture success rate is relatively high.

Description

technical field [0001] The present disclosure relates to the technical field of biomedicine, and in particular to a method for culturing undifferentiated or undifferentiated thyroid cancer organoids, a thyroid cancer medium for culturing undifferentiated or undifferentiated thyroid cancer organoids, and undifferentiated or undifferentiated thyroid cancer organoids. Thyroid cancer organoids. Background technique [0002] Thyroid cancer is a malignant tumor originating from thyroid follicular and parafollicular epithelial cells, including differentiated thyroid cancer and undifferentiated thyroid cancer. [0003] Differentiated thyroid carcinoma (DTC) accounts for 90% of all thyroid cancers, including papillary thyroid carcinoma (PTC) and follicular thyroid carcinoma (FTC). DTC tumor cells have the iodine uptake ability of normal thyroid cells, which can be combined with surgical resection 131 I radiotherapy and thyroid hormone instead of suppressive therapy, so the prognosi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09
CPCC12N5/0617C12N5/0693C12N2500/32C12N2500/38C12N2501/11C12N2513/00
Inventor 李程黄思颖李堃孙志坚康平
Owner 北京科途医学科技有限公司
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