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Preparation method of pogostemon cablin hydrolat and in-vitro antioxidant activity evaluation method of pogostemon cablin hydrolat

A technology of antioxidant activity and evaluation method, applied in the field of preparation of hand incense hydrosol, can solve the problem of lack of research on the evaluation of anti-oxidation of incense hydrosol, and achieve the effects of improving environmental odor, simple operation, and scavenging oxygen free radicals

Inactive Publication Date: 2021-01-12
梵诗吉生物科技(东莞)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no domestic research on the preparation of Shouxiang Hydrosol and its in vitro antioxidant evaluation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A preparation method of hand fragrance pure dew, comprising the following operations:

[0046] 1) Take fresh incense sticks, crush them into fragments of a certain volume, wash them, and drain the water;

[0047] 2) Place the drained incense in the distiller, add a certain volume of distilled water, turn on the circulating water, heat, gradually turn from low power to high power, and collect the distillate. Stop collecting when the weight of the incense is 0.8 to 1.5 times, and obtain the hand incense hydrosol, which is stored in a refrigerator at 4°C.

Embodiment 2

[0049] An in vitro antioxidant activity evaluation method of Zhixiang Hydrosol, comprising the following operations:

[0050] 1) According to the method of Example 1, hand fragrance hydrosol is prepared, and it is set as the experimental group;

[0051] 2) Set the DPPH free radical scavenging activity experiment as the in vitro antioxidant activity experiment, and set the vitamin C solution as the control group;

[0052] 3) In vitro antioxidant activity experiments were carried out on the experimental group and the control group respectively, and the specific experimental methods were as follows:

[0053] Accurately weigh 0.00491g DPPH, dissolve it with ethanol, dilute it into a 100ml volumetric flask, draw 2ml of sample solutions of different concentrations and place them in a 5ml centrifuge tube, add 2ml of DPPH solution, shake well, let stand for 30min, and measure at 517nm The absorbance value is denoted as A1;

[0054] Add the absorbance value of the DPPH solution mixture...

Embodiment 3

[0060] An in vitro antioxidant activity evaluation method of Zhixiang Hydrosol, comprising the following operations:

[0061] 1) According to the method of Example 1, hand fragrance hydrosol is prepared, and it is set as the experimental group;

[0062] 2) Set the reducing power test as the in vitro antioxidant activity test, set the vitamin C solution as the control group, set ethanol as the blank group, and operate synchronously;

[0063] 3) In vitro antioxidant activity experiments were carried out on the experimental group, control group and blank group respectively. The specific experimental methods are as follows:

[0064] Take 1ml of the sample and place it in a 10ml centrifuge tube, add 2.0ml of 0.2mol / LPH6.6 phosphate buffer solution and 2.0ml of potassium ferricyanide solution with a mass fraction of 1%, vortex and mix, then heat in a water bath at 50°C for 20min , ice-water bath for 5 minutes, let cool to room temperature, add 2.0ml of 10% trichloroacetic acid solu...

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PUM

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Abstract

The invention belongs to the technical field of natural medicines, and particularly relates to a preparation method of pogostemon cablin hydrolat and an in-vitro antioxidant activity evaluation methodof the pogostemon cablin hydrolat, and the preparation method comprises the following steps: taking fresh pogostemon cablin, crushing into fragments, cleaning, and draining off water; and putting thedrained hand incense into a distiller, adding distilled water, heating, gradually converting the power of the distiller from low power to high power, and collecting distillate to obtain the pogostemon cablin hydrolat. The pogostemon cablin hydrolat prepared by adopting the method disclosed by the invention is simple and convenient to operate, and the defect that the preparation method of the pogostemon cablin hydrolat is blank in China is filled. The pogostemon cablin hydrolat has a certain antioxidant activity, and the antioxidant serving as an exogenous antioxidant can effectively remove oxygen free radicals, so that the skin achieves the effects of aging resistance, whitening, sun protection, moisturizing and the like. According to the invention, several in-vitro antioxidant activity experimental methods are adopted to evaluate the antioxidant activity of the pogostemon cablin hydrolat, and the method has the characteristics of simplicity and accuracy in operation.

Description

technical field [0001] The invention belongs to the technical field of natural medicines, and in particular relates to a preparation method and an in vitro anti-oxidation activity evaluation method of Shouxiang Hydrosol. Background technique [0002] Zhishouxiang, also known as Zuoshouxiang and Mint, is a plant of the Labiatae family. It is the most spice and medicinal plant in foreign countries. At present, the domestic research on the chemical structure of Dianxiang mainly focuses on the components of the volatile oil of Dianxiang. From the volatile oil of Dianxiang, carvacrol (57.82%), thymol (15.24%), caryophyllene oxide (1.13%), There are 34 chemical components such as terpinene (10.01%), ρ-cymene (5.65%), and α-caryophyllene (2.32%). In the acute toxicity experiment of the volatile oil of Xiangshouxiang, it was shown that the volatile oil of Xiangshouxiang has certain acute toxicity to mice. [0003] Pure dew, also known as water essential oil, refers to a 100% satur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/02A61K8/9789A61Q17/04A61Q19/02A61Q19/08G01N21/31
CPCC11B9/02C11B9/027A61K8/9789A61Q19/08A61Q19/02A61Q17/04G01N21/31
Inventor 丁鸿燕陈进丁吴铁杨泳诗胡佩诗
Owner 梵诗吉生物科技(东莞)有限公司
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