miRNA data and transcriptome data penetration analysis method and system

Abstract

The invention discloses a miRNA data and transcriptome data penetration analysis method and system, and the method comprises the following steps: S1, carrying out the small RNA sequencing of a sample,carrying out the biological information analysis of the small RNA sequencing data, and performing screening to obtain differentially expressed miRNAs according to the expression quantity of the miRNAs; S2, performing transcriptome sequencing on the sample, performing bioinformation analysis on transcriptome sequencing data, and obtaining differentially expressed mRNA through screening according to the expression quantity of the mRNA; S3, selecting differentially expressed miRNA and differentially expressed mRNA obtained by small RNA sequencing and transcriptome sequencing to perform subsequent correlation analysis, thereby realizing a penetration analysis technology suitable for miRNA data and transcriptome sequencing data of any organism, effectively improving the data utilization rate,and improving the accuracy of data analysis. Meanwhile, potential relations between data and biological information with important research value are mined from different levels, and the purpose of data integration is achieved.

Description

technical field [0001] The present invention relates to the technical field of miRNA and transcriptomics, in particular to a method and system for through-analysis of miRNA data and transcriptome data. Background technique [0002] Transcriptome is defined as the collection of all RNA (transcripts) transcribed by a specific species, tissue or cell type, including mRNA and non-coding RNA (non-coding RNA); non-coding RNA mainly refers to microRNA (miRNA). [0003] MicroRNA (miRNA) is a kind of endogenous non-coding RNA with a length of about 19-25nt, which is widely involved in the post-transcriptional regulation of genes, and has a high degree of sequence conservation, expression timing and tissue specificity. Studies have shown that miRNAs participate in various regulatory pathways, including development, virus defense, hematopoietic process, fat metabolism, etc., and play an important role in gene expression regulation. The process of mRNA translation into peptide chains i...

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Problems solved by technology

[0004] With the continuous deepening of research, the biological information reflected by a single omics data has certain limitations, and cannot fully and deeply achieve the expected research purpose; therefore, it is necessary to combine multiple omics technologies for data integration to comprehensively and deeply Scientific exploration; the Chinese patent with the publication number CN 104911261 B discloses a method for studying the interaction mode of rice and pathogens, and comprehensively applies transcriptomics, proteomics and miRNA-omics to study the response of rice to pathogen infection method of interactive mode

There is still a lack of a systematic and complete method that can be applied to the analysis of miRNA and transcriptomics in any species

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