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Inoculant carrier, preparation method and application thereof and preparation method for lactobacillus solid inoculant

A technology of solid bacterial agent and lactic acid bacteria, applied in the field of microorganisms, can solve the problems of loss of lactic acid, short storage period, waste of supernatant, etc., and achieve the effect of efficient production and reduction of waste water.

Inactive Publication Date: 2021-01-05
COFCO BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] With the application and demand of the fermented feed industry, it is imperative to increase the production of lactic acid bacteria fermented feed, but the preparation process of the liquid seed solution is complicated and the storage period is short; the storage period of the freeze-dried bacterial agent is long, but the centrifuged supernatant is wasted , not only produces waste water but also loses the high concentration of lactic acid

Method used

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  • Inoculant carrier, preparation method and application thereof and preparation method for lactobacillus solid inoculant
  • Inoculant carrier, preparation method and application thereof and preparation method for lactobacillus solid inoculant

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preparation example Construction

[0025] Another aspect of the present invention provides a method for preparing the bacterial agent carrier as described above, the method comprising: mixing calcium carbonate, crude fiber raw material powder, starch and montmorillonite to obtain the bacterial agent carrier.

[0026] In the present invention, the types and contents of calcium carbonate, crude fiber raw material powder, starch and montmorillonite involved in the preparation method of the above-mentioned bacterial agent carrier are all described in detail in the first aspect of the present invention, and will not be repeated here. repeat.

[0027] In the present invention, as long as the above components are directly mixed to prepare the bacterial agent carrier described in the present invention, and applying it to the preservation of the bacterial agent can effectively prolong the storage period of the prepared solid bacterial agent, In the case of preferably preparing the bacterial agent carrier according to th...

preparation example 1

[0066] This preparation example is used to illustrate the preparation method of lactic acid bacteria fermentation broth

[0067] 1) Strain activation: the Lactobacillus plantarum stored in an ultra-low temperature refrigerator was naturally thawed 0.5 h in advance, and inoculated into MRS broth medium with an inoculum volume of 0.2% by volume.

[0068] Culture conditions: static culture at 35°C. When the OD value grows to 6-7 and the pH is 4.0, the primary seed shake flask is finished.

[0069] 2) Secondary shake flask fermentation: the culture medium is MRS broth medium, the shake flask inoculum size: 0.2% by volume;

[0070] Culture conditions: static culture, temperature 35°C.

[0071] The OD value grows to 12-15, the pH is around 5.0, and the secondary seed shake flask is over.

[0072] 3) Expanded tank fermentation

[0073] The medium is MRS broth medium, and the inoculation amount is 0.2% by volume; the culture condition is static culture, and the temperature is 37°C...

Embodiment 1

[0076] This embodiment is used to illustrate the preparation method of the lactic acid bacteria solid microbial agent of the present invention

[0077] 1) After mixing 8g of calcium carbonate and 67g of rice husk powder evenly, measure and adjust the pH to 8.5 to obtain the first mixture;

[0078] 2) after the first mixture is mixed with 10g feed grade montmorillonite powder, the second mixture is obtained;

[0079] 3) The second mixture was uniformly mixed with 15 g of cornstarch, and the pH was measured and adjusted to 7 to obtain the bacterial agent carrier.

[0080] 4) Add 10 g of skim milk powder directly to 50 g of lactic acid bacteria fermentation broth and mix for 60 minutes to obtain a mixed bacterial agent.

[0081] 5) The bacterial agent carrier was added to the mixed bacterial agent and mixed, put into an oven for drying at a drying temperature of 35° C. and a drying time of 1 hour to obtain a solid lactic acid bacteria bacterial agent A1.

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Abstract

The invention relates to the technical field of microorganisms, and discloses an inoculant carrier, a preparation method and application thereof and a preparation method for a lactobacillus solid inoculant. The inoculant carrier contains calcium carbonate, crude fiber raw material powder, starch and montmorillonite. Besides the inoculant carrier and lactobacillus, the lactobacillus solid inoculantfurther contains a thallus protective agent. Compared with the prior art, the inoculant carrier disclosed by the invention has a nutrient slow release property; due to the metabolic feedback effect of alkaline substances in the inoculant carrier on high-concentration lactic acid and the protective property of the thallus protective agent of the prepared lactobacillus solid inoculant, lactobacillus thalli can be slowly multiplied in the earlier stage of storage, so that the number of the thalli is not lower than an initial value after the thalli are stored for 6 months; and meanwhile, fermentation liquor is completely utilized in an inoculant preparation process, so that green and efficient production of the lactobacillus inoculant is realized.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a bacterial agent carrier, a preparation method and application thereof, and a preparation method of a lactic acid bacteria solid microbial agent. Background technique [0002] At present, many countries attach great importance to the research of lactic acid bacteria. It not only plays a role in food production, disease prevention and biological preservation, but also has a wide range of applications in animal production, especially in fermented feed, where lactic acid bacteria are most widely used. Lactic acid bacteria fermented feed can produce its unique fermented sour aroma, which can not only improve the palatability of animals to feed, improve the production performance of animals, maintain the balance of flora in the intestinal tract of animals, but also have a certain immune regulation activity. Significance. [0003] With the application and demand of the ferment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12N11/12C12N11/10C12N1/20C12N1/04
CPCC12N11/14C12N11/12C12N11/10C12N1/20C12N1/04
Inventor 周勇邴狄祥佟毅朱光耀郑晓卫金渭武卢宗梅
Owner COFCO BIOTECHNOLOGY CO LTD
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