Plant lactobacillus solid inoculant, preparation method thereof and application of plant lactobacillus solid inoculant
A technology of plant lactic acid bacteria and solid microbial agents, applied in biochemical equipment and methods, methods based on microorganisms, applications, etc., can solve the problems of low bacterial count, low metabolite lactic acid content, poor fermentation effect, etc.
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preparation example Construction
[0020] First aspect, the present invention provides a kind of preparation method of plant lactic acid bacteria solid microbial agent, the method comprises:
[0021] (1) inoculating the strains of plant lactic acid bacteria into the first solid fermentation medium to carry out the first fermentation culture to obtain the first fermentation material;
[0022] (2) inoculating the first fermentation material into a second solid fermentation medium to carry out a second fermentation culture to obtain a second fermentation material;
[0023] Wherein, the first solid fermentation medium contains starchy raw material crushed product and starch residue;
[0024] The second solid fermentation medium contains DDGS, DDS, starchy raw material skin and calcium source.
[0025] According to the present invention, although the strains of plant lactic acid bacteria are inoculated into the above specific solid fermentation medium for secondary culture, the lactic acid content and the cell dens...
preparation example 1
[0067] This preparation example is used to illustrate the preparation of the bacterial classification of plant lactic acid bacteria
[0068] According to the medium and conditions in Table 1, the activation and expansion of plant lactic acid bacteria were carried out, and the fermentation liquid was obtained as the inoculum with the expansion tank.
[0069] Table 1
[0070]
preparation example 2
[0072] This preparation is used to illustrate the activation of Saccharomyces cerevisiae inoculum
[0073] Activate the yeast agent with 7 times of 2% by weight aqueous glucose solution at 30-35°C for 15-30 minutes to obtain activated yeast
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