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Indel markers associated with rose petals and molecular methods for identification of rose petals

A technology of red and petals, applied in biochemical equipment and methods, microbiological determination/inspection, DNA/RNA fragments, etc., to achieve the effects of easy promotion, early seedling breeding, and simple operation techniques

Active Publication Date: 2022-05-06
北京市林业果树科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the DNA level, how to distinguish rose petals with red petals, there are no relevant reports on the techniques and methods in this regard

Method used

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  • Indel markers associated with rose petals and molecular methods for identification of rose petals
  • Indel markers associated with rose petals and molecular methods for identification of rose petals
  • Indel markers associated with rose petals and molecular methods for identification of rose petals

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1 verifies the petal color of Yueyuehong and Tiantanbai with the method provided by the invention

[0037] The leaves of Chinese rose varieties Yueyuehong and Tiantanbai were taken as identification materials. The petals of Yueyuehong are red, and the petals of Tiantanbai are white, such as figure 1 shown.

[0038] The specific operation is as follows:

[0039] 1. Use the plant genomic DNA extraction kit DP360 to extract the genomic DNA of the leaves of Yueyuehong and Tiantanbai respectively. The extraction steps are operated according to the kit instructions. After extraction, electrophoresis identification is carried out. The results are as follows figure 2 shown.

[0040] 2. Using the extracted genomic DNA as a template, use the upstream primer SEQ ID No.3 and the downstream primer SEQ ID No.4 to perform PCR reaction amplification using a PCR amplification kit;

[0041] The upstream primer sequence (5'→3') SEQ ID No.3 is GTTGGTGCACCAGATCAAACAATTGGTGC;...

Embodiment 2

[0051] Example 2 Use the method of the present invention to verify 18 materials of the hybrid progeny population of Yueyuehong and Tiantanbai

[0052] Carry out hybridization with Yueyuehong (female parent) and Tiantanbai (male parent) in Example 1 as parents, take 18 different progeny plants in the hybrid progeny group to distinguish and verify, and then check with the real petal color of the above-mentioned materials in the field fake.

[0053] 1. Use the Plant Genomic DNA Extraction Kit DP360 to extract the genomic DNA (concentration: 20 ng / μL) of the leaves of the above-mentioned 18 different offspring plants (numbered as material 1 to material 18), and extract according to the steps provided in the kit instructions;

[0054] 2. Using the extracted genomic DNA from materials 1 to 18 as templates, use the upstream primer SEQ ID No.3 and the downstream primer SEQ ID No.4 to perform PCR reaction amplification using a PCR amplification kit;

[0055] The upstream primer sequen...

Embodiment 3

[0062] Embodiment 3 uses the method of the present invention to identify 17 different-colored rose varieties

[0063] 17 rose varieties of different colors were taken for blind testing, and the results of Indel molecular testing were used to speculate whether the flower color of the rose was red, and then the real petal colors of the above-mentioned rose varieties in the field were used to check for counterfeit.

[0064] Proceed as follows:

[0065] 1. Use the plant genomic DNA extraction kit to extract the leaf genomic DNA of 17 materials (numbered as material 1 to material 17), and the extraction is carried out according to the kit instructions;

[0066] 2. Using the extracted genomic DNA of the leaves of materials 1 to 17 as templates, use the upstream primer SEQ ID No.3 and the downstream primer SEQ ID No.4 to perform PCR reaction amplification using a PCR amplification kit;

[0067] The upstream primer sequence (5'→3') SEQ ID No.3 is GTTGGTGCACCAGATCAAACAATTGGTGC;

[00...

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Abstract

The invention provides an Indel marker related to the red rose petal and a molecular method for identifying the red rose petal. The nucleotide sequence of the Indel tag is shown in SEQ ID No.1. The molecular method for identifying the red rose petals includes separating and extracting the genome DNA of the rose material to be identified; using Indel-marked specific primers to carry out PCR and electrophoresis to observe whether there is an Indel band. When there are Indel bands, the variety is a red rose; without Indel bands, the variety is not a red rose, and may be a white, green or yellow rose. The invention can quickly distinguish pre-flowering red and non-red rose varieties through the unique Indel markers of rose petals, serve rose flower color molecular assisted breeding, and especially provide technical support for rapid selection of rose flower color at seedling stage.

Description

technical field [0001] The invention belongs to the field of agricultural breeding, and relates to a marker and method for identifying red rose petals, in particular to an Indel marker related to red rose petals and a molecular method for identifying red rose petals. Background technique [0002] Rose (Rosa L.), belonging to Rosaceae Rosa, is one of the important woody ornamental flowers. It is also one of the most important cut flowers and potted flowers in the world. Roses are rich in flower colors, including red, white, green, yellow, orange, pink, blue, multi-color, etc. The flower colors are mainly determined by anthocyanins, carotenoids and chlorophyll, among which the red rose petals are mainly determined by anthocyanins . It is a water-soluble pigment that exists in specific tissues and cells in plants, such as the vacuoles of plant cells, and is a secondary metabolite produced by plants. At the molecular level, anthocyanins are regulated by regulatory genes and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12Q1/686C12N15/11
CPCC12Q1/6895C12Q1/686C12Q2600/156C12Q2600/13
Inventor 金万梅李茂福杨媛王华张宏范又维孙佩
Owner 北京市林业果树科学研究院
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