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circRNA of targeted competitive binding of oar-miR-29b and application of circRNA

A combination of competition and targeted technology, applied in the field of genetic engineering, can solve problems such as lack of supplementary feeding, late sexual maturity, long development cycle, etc., and achieve the effect of enriching data

Pending Publication Date: 2021-01-01
GANSU AGRI UNIV
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Problems solved by technology

However, due to its living environment, long-term grazing and lack of supplementary feeding, it has the characteristics of long development cycle, late sexual maturity, and low fecundity. It is an ideal breed for exploring the regulation mechanism of sheep testis development and function maintenance.

Method used

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  • circRNA of targeted competitive binding of oar-miR-29b and application of circRNA
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  • circRNA of targeted competitive binding of oar-miR-29b and application of circRNA

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Embodiment Construction

[0018] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0019] 1. Experimental animals and sample collection

[0020] In the present invention, healthy male Tibetan sheep from 3 months old (immature), 1 year old (sexually mature) and 3 years old (adult) are used as experimental animals, and all test sheep have similar genetic backgrounds (same male parent, different female parent) , Feed ad libitum without supplementary feeding under the conditions of grazing fences in natural pastures at an altitude of 3000-3500m. After the test sheep were slaughtered, the right testis tissue of each animal was quickly collected and the albuginea was peeled off, cut i...

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Abstract

The invention discloses circRNA of target competitive binding of oar-miR-29b and an application of the circRNA. Healthy male Tibetan sheep testicular tissues in different development stages are used as test materials, circular RNA in testis is identified and analyzed through an RNA-seq technology and a related bioinformatics analysis method so as to dig the circRNA related to testis development and function maintenance, the circRNA is circ_024949 and / or circular RNA circ_026259, the nucleotide sequence of the circRNA circ_024949 is shown as SEQ ID No:1, and the nucleotide sequence of the circular RNA circ_026259 is shown as SEQ ID No:2. On the basis, the invention discloses the application of the circRNA as a marker for detecting the testis function or development condition of Tibetan sheep, to preparation of a medicine for regulating and controlling the testis function or development of the Tibetan sheep and to expression analysis or research of a downstream target gene BOLL. The circular RNA circ_029155 can be used for detecting and evaluating the testis development and spermatogenesis functions of the sheep, and lays a foundation for further exploring a molecular action mechanism of the testis development and spermatogenesis of the sheep and subsequent application and development.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a circRNA targeting competitively binding oar-miR-29b and its application. Background technique [0002] As an important part of the reproductive and endocrine system, the testis mainly functions to produce male gametes (sperm) and sex hormones, and is a reproductive organ necessary to maintain the normal reproductive function of adult male animals. The testis has the most diverse transcriptome, including coding RNA (mRNA) and non-coding RNA (such as circRNA, lncRNA and miRNA), which participate in the regulation of testicular development and spermatogenesis through highly synergistic effects. Circular RNA (circRNA), as a type of endogenous non-coding RNA with a closed loop structure and abundantly expressed in eukaryotes, can act as a miRNA sponge to regulate the expression and function of downstream genes. However, at present, the application of circRNA...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12Q1/6883A61K31/7105A61P15/08
CPCC12N15/113C12Q1/6883A61P15/08C12N2310/532C12N2310/113C12Q2600/158C12Q2600/178
Inventor 李讨讨马友记雒瑞瑞王慧慧张勇赵兴绪
Owner GANSU AGRI UNIV
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